| VM、VEGF在子宫内膜癌中表达的意义及预后影响 |
| 投稿时间：2017-09-29 修订日期：2017-10-12 点此下载全文 |
| 引用本文：李美艳.VM、VEGF在子宫内膜癌中表达的意义及预后影响[J].医学研究杂志,2018,47(6):93-98,103 |
| DOI：
10.11969/j.issn.1673-548X.2018.06.022 |
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| 基金项目:河北省科技厅科技项目（162777198） |
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| 中文摘要:目的 检测子宫内膜癌（endometrial carcinoma，EC）组织标本中是否存在血管生成拟态（vasculogenic mimicry，VM），探讨VM与血管内皮生长因子（vascular endothelial growth factor，VEGF）在EC中的表达情况及关系，并讨论二者与患者术后生存之间的关系。方法 采用CD34-PAS双重染色法，检测EC组织、不典型增生和正常子宫内膜组织中VM的表达，免疫组织化学SP法检测VEGF蛋白的表达情况，并分析二者与EC患者临床病理因素及术后生存之间的关系。结果 VM和VEGF的阳性表达率在EC组织中分别为39.59%和68.02%，在不典型增生子宫内膜中分别为4.71%和21.18%，在正常子宫内膜中分别为1.59%和4.76%，二者表达在EC组较其他组差异均有统计学意义（χ2=60.668，P<0.05；χ2=98.976，P<0.05）。VM和VEGF蛋白均与手术-病理分期、组织学分化有关（P<0.05），二者表达呈正相关关系（r=0.293，P=0.000）。EC患者预后的Kaplan-Meier单因素分析显示，VM阳性和VEGF过表达患者5年总体生存率分别为66.67%和79.10%，低于VM阴性和VEGF低表达患者的95.80%和95.24%（P均<0.05）。EC患者预后的Cox多因素分析显示，手术-病理分期、组织学分化、淋巴结转移、VM阳性和VEGF过表达是影响EC预后的显著因素。结论 EC组织标本中存在VM，联合检测VM、VEGF对EC患者侵袭、转移及预后判断有重要意义，二者可能作为诊断和评估EC预后的参考指标。 |
| 中文关键词:子宫内膜癌 血管生成拟态 VEGF蛋白 预后 |
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| Expression and Prognostic Significance of VM and VEGF in Endometrial Carcinoma |
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| Abstract:Objective To detect the presence of vasculogenic mimicry (VM) in endometrial carcinoma (EC) tissue specimens, and to investigate the expression and relationship between VM and vascular endothelial growth factor (VEGF) in EC, and discuss the relationship between the two and the survival of patients after surgery. Methods The expression of VM in EC tissue, atypical hyperplasia and normal endometrial tissue was detected by CD34-PAS double staining. The expression of VEGF protein was detected by immunohistochemical SP method, and the relationship between the clinicopathological factors and the survival of EC patients was analyzed. Results The positive expression rates of VM and VEGF were 39.59% and 68.02% in EC tissues, 4.71% and 21.18% in atypical hyperplasia endometrium respectively, 1.59% and 4.76% in normal endometrium respectively, the expression of the two in the EC group were statistically significant compared with the other groups(χ2=60.668,P<0.05;χ2=98.976,P<0.05). Both VM and VEGF protein were associated with surgical-pathological stage and histological differentiation (P<0.05), positive correlation was found between them (r=0.293, P=0.000). Kaplan-Meier univariate analysis of the prognosis of patients with EC showed that the 5-year overall survival rates of VM-positive and VEGF-positive patients were 66.67% and 79.10% respectively, which were lower than 95.80% and 95.24% in VM negative and VEGF negative patients(P all <0.05). Cox multivariate analysis of prognostic factors in EC patients showed that surgical-pathological stage, histological differentiation, lymph node metastasis, VM positive and overexpression of VEGF were significant factors influencing EC prognosis. Conclusion VM was present in EC tissue specimens. Combined detection of VM and VEGF plays an important role in the invasion, metastasis and prognosis of EC patients, both of which may serve as a reference for the diagnosis and evaluation of EC prognosis. |
| keywords:Endometrial carcinoma Vasculogenic mimicry VEGF protein Prognosis |
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