IL-10启动子区基因多态性与神经梅毒的相关性研究
投稿时间:2017-08-07  修订日期:2017-08-31  点此下载全文
引用本文:罗涟,郭莉丽,李昆,周佳君.IL-10启动子区基因多态性与神经梅毒的相关性研究[J].医学研究杂志,2018,47(6):139-143
DOI: 10.11969/j.issn.1673-548X.2018.06.032
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作者单位E-mail
罗涟 310023 杭州市西溪医院神经内科  
郭莉丽 310023 杭州市西溪医院神经内科  
李昆 310023 杭州市西溪医院神经内科  
周佳君 310023 杭州市西溪医院神经内科 tgctrgvtrvh@qq.com 
基金项目:浙江省杭州市卫生科技计划项目(2017A45)
中文摘要:目的 研究白细胞介素-10(IL-10)基因启动子区的多态性与神经梅毒发病风险的相关性。方法 随机选择2013年4月~2016年8月期间笔者医院收治的150例梅毒患者作为研究组,其中包括48例神经梅毒患者和102例非神经梅毒患者,另招募同期在笔者医院门诊处的健康体检者150例作为对照组。采用Sanger测序法检测IL-10基因启动子中-592和-1082位点的SNP基因型,并分析-592和-1082位点的基因型与IL-10水平和神经梅毒发病风险之间的相关性。结果 神经梅毒患者的CSF中IL-10水平明显比非神经梅毒患者高(7.94pg/ml vs 0.71pg/ml),差异有统计学意义(P<0.05)。研究组患者与对照组IL-10启动子区-592位点各基因型各等位基因频率和-1082位点的G、A等位基因频率以及单倍体G/C和A/A频率之间的差异均有统计学意义(P<0.05)。神经梅毒组患者IL-10启动子区-592位点的CC基因型频率和-1082位点的GG基因型频率明显比非神经梅毒组患者高,差异有统计学意义(P<0.05)。-1082位点GG基因型携带者患有神经梅毒的风险明显高于其他基因型携带者(OR=10.21,95% CI:1.94~53.04,P=0.001)。非神经梅毒组的患者中1082位点AA基因型频率明显高于梅毒患者组,差异有统计学意义(P<0.05)。-592位点CC基因型的携带者患神经梅毒的风险明显高于其他基因型携带者(OR=7.65,95% CI:1.38~42.55,P=0.004)。-1082/-592 G/C单倍体具有很高的神经梅毒患病风险(OR=45.14,95% CI:2.34~421.15,P=0.000)。-1082位点GG基因型的患者的CSF中IL-10水平明显比A等位基因携带者高,差异有统计学意义(8.15pg/ml vs 0.81pg/ml,P=0.000)。-592位点CC基因型、CA基因型和AA基因型患者CSF中的IL-10水平之间的差异无统计学意义(1.24pg/ml、1.00pg/ml和0.82pg/ml,P>0.05)。结论 IL-10基因启动子区的多态性与神经梅毒发病风险相关,携带-592C和-1082G等位基因的患神经梅毒的风险更高,脑脊液中IL-10水平也更高。
中文关键词:白细胞介素-10  单核苷酸多态性  神经梅毒  梅毒
 
Association between Interleukin-10 Promoter Region Gene Polymorphism with Neurosyphilis
Abstract:Objective To study the correlation between the polymorphism of interleukin-10 (IL-10) gene promoter region and the risk of neurosyphilis. Methods A total of 150 syphilis patients were enrolled in our hospital from April 2013 to August 2016 as the study group, including 48 neurosyphilis patients and 102 non-neurosyphilis patients. Another 150 health subjects were recruited as control group. SNP genotypes of -592 and -1082 loci in IL-10 gene promoter were detected by Sanger sequencing and the correlation between genotype at -592 and -1082 loci with IL-10 level and the risk of neurosyphilis were analyzed. Results The level of IL-10 in CSF of neurosyphilis patients was significantly higher than that in non-neurosyphilis patients (7.94pg/ml vs 0.71pg/ml), the difference was statistically significant (P<0.05). The differences of allele frequencies of each genotype in IL-10 promoter region -592 locus, the G and A allele frequencies and the haploid G/C and A/A gene frequencies between the study group and the control were statistically significant (P<0.05). The frequency of CC genotype in IL-10 promoter region -592 locus and the frequency of GG genotype in the IL-10 promoter -1082 site of neurosyphilis group were significantly higher than those in the non-hypertensive group (P<0.05). The risk of neurosyphilis was significantly higher in -1082 locus GG genotype carriers than in other genotype carriers(OR=10.21,95%CI:1.94-53.04,P=0.001). The frequencies of AA genotype in 1082 locus in neurosyphilis patients were significantly higher than those in syphilis group (P<0.05). The risk of neurosyphilis was significantly higher in carriers of CC genotype at -592 locus than in other genotype carriers (OR=7.65,95%CI:1.38-42.55,P=0.004). -1082/-592 G/C haplotypes had a high risk of neurosyphilis (OR=45.14, 95%CI:2.34-421.15, P=0.000). The level of IL-10 in the CSF of GG genotype in -1082 locus was significantly higher than that of an allele carrier (8.15pg/ml vs 0.81pg/ml, P=0.000). There was no significant difference in IL-10 levels between CC genotype, CA genotype, and AA genotype in CSF (1.24pg/ml, 1.00pg/ml and 0.82pg/ml, P>0.05). Conclusion The polymorphism of IL-10 gene promoter region is associated with the risk of neurosyphilis, -592C and -1082G allele carriers have a higher risk of developing neurosyphilis and higher levels of IL-10 in cerebrospinal fluid.
keywords:Interleukin-10  Single nucleotide polymorphisms  Neurosyphilis  Syphilis
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