| 新型查尔酮FMC对大鼠局灶性脑缺血再灌注损伤的保护作用 |
| 投稿时间：2017-10-10 修订日期：2017-10-25 点此下载全文 |
| 引用本文：梁海,吴久鸿.新型查尔酮FMC对大鼠局灶性脑缺血再灌注损伤的保护作用[J].医学研究杂志,2018,47(7):43-47 |
| DOI：
10.11969/j.issn.1673-548X.2018.07.010 |
| 摘要点击次数: 819 |
| 全文下载次数: 722 |
|
| 基金项目:国家自然科学基金资助项目（81072546、81470156） |
|
| 中文摘要:目的 研究新型查尔酮（FMC）（3'-甲酰基-4'，6'-二羟基-2'-甲氧基-5'-甲基查尔酮）对大鼠局灶性脑缺血再灌注损伤的保护作用。方法 建立重复性好、梗死部位恒定、成功率高的局灶性脑缺血再灌注损伤模型，以水溶性的FMC溶液腹腔注射，观察FMC对脑缺血大鼠神经行为、脑梗死范围以及脑组织的含水量、超氧化歧化酶（SOD）、丙二醛（MDA）的影响。结果 术后出现追尾征及特殊体态，于2h后大脑中动脉供血区出现缺血。脑组织TTC染色均匀，出现梗死的脑组织面积相对于全脑百分比为27.96%±9.45%，动物模型成功率为78.6%。FMC组的脑含水量、MDA显著降低，SOD显著升高，梗死率降低。结论 本方法构成局灶性缺血再灌注模型，操作简单，重复性好，大鼠体重和年龄、麻醉剂应用、栓线直径和插入深度、控制出血和术后护理等是模型制作成功的关键因素。FMC可能通过降低脑组织水含量、MDA水平，升高SOD水平，减少梗死体积，对缺血再灌注损伤的脑组织起到一定的保护作用。 |
| 中文关键词:线栓法 局灶性 脑缺血再灌注损伤 FMC MDA SOD |
| |
| Protective Effect of Novel Chalcone FMC on Focal Cerebral Ischemia Reperfusion Injury in Rats |
|
|
| Abstract:Objective To study on the protective mechanism of FMC on focal cerebral ischemia-reperfusion injury in rats. Methods The model of focal cerebral ischemia-reperfusion injury with favorable reproducibility, constantly infarction location and high success rate were induced by MACO. We studied on the effect of FMC on neural behavior, water content, SOD, MDA and infarction volume in the brain tissue of focal cerebral ischemia-reperfusion injury by intraperitoneal injection of water-soluble FMC solution. Results After MCAO, special neurological syndromes were showed by rats, the infarct surface in the area of MCA (TTC coloring, after MCAO 2h). The percentage of the infarct area was (27.96±9.45)%. The model success rate was 78.6%. Conclusion A reversible model of regional cerebral ischemia in rats with simple operation and higher reproducibility was developed. There are key points to build a successful model such as rat's weight and age, anesthetic application, suture diameter, depth of penetration, control of hemorrhage and postoperative care. The protection of FMC on cerebral tissue was played by reducing the cerebral water content, the level of MDA, the infarct volume and by increasing the level SOD. |
| keywords:Cerebral ischemia reperfusion Focal Ischemia reperfusion injury FMC MDA SOD |
| 查看全文 查看/发表评论 下载PDF阅读器 |
