| 疡愈涂剂对免疫抑制小鼠伤口愈合和IL-1β、TNF-α的影响 |
| 投稿时间：2017-11-10 修订日期：2017-11-29 点此下载全文 |
| 引用本文：何秀娟,张金超,林燕,李萍.疡愈涂剂对免疫抑制小鼠伤口愈合和IL-1β、TNF-α的影响[J].医学研究杂志,2018,47(9):29-33 |
| DOI：
10.11969/j.issn.1673-548X.2018.09.008 |
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| 基金项目:国家自然科学基金资助项目（81774312，81403270，81774328，81403409） |
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| 中文摘要:目的 研究疡愈涂剂对免疫抑制小鼠皮肤伤口愈合作用和对创面局部炎性反应的影响。方法 以氢化可的松处理小鼠造成免疫抑制状态，采取全厚皮切除术，复制愈合迟缓伤口模型。随机分为对照组、模型组和疡愈涂剂高、中、低浓度组。治疗组伤口外用疡愈涂剂，一次性无菌敷料贴包扎，隔日换药。观察小鼠创伤后3、7和10天伤口面积； HE染色观察创面的组织学变化；放免法检测创面白细胞介素1（IL-1β）和肿瘤坏死因子α（TNF-α）水平。结果 用药后7天和10天与模型组比较，疡愈涂剂能够明显缩小免疫抑制小鼠创面面积（P<0.01，P<0.05），组织学结果显示疡愈涂剂促进创面成纤维细胞增殖和血管生成。用药后3天时疡愈涂剂各浓度组IL-1β水平明显高于模型组（P<0.01），7天时无明显差异，10天时高、中浓度组明显下降（P<0.01，P<0.05）。与模型组比较，疡愈涂剂各浓度组3天时TNF-α水平差异无统计学意义，7天时明显升高，10天时疡愈涂剂中、低浓度组明显下降（P<0.01）。结论 疡愈涂剂能促进免疫抑制小鼠皮肤伤口愈合，其机制可能是早期促进创面炎性反应来启动创面的修复，中晚期抑制炎性反应，促进肉芽组织生成。 |
| 中文关键词:伤口愈合 疡愈涂剂 慢性皮肤溃疡 |
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| Effects of YYTJ on Wound Healing and IL-1β,TNF-α Level of Immuno-suppressive Mice |
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| Abstract:Objective To study the healing effect of YangYuTuJi (YYTJ) on skin wounds of immune-suppressive mice and its effect on the local inflammatory response of wounds. Methods Mice were treated with hydrocortisone to induce immune-suppression. Full-thickness skin resection was performed to replicate the delayed-healing wound model. 150 animals were randomly divided into control, model groups and YYTJ groups with high/medium/low dose. The YYTJ groups were externally treated with YYTJ. The model and control groups were both treated with N.S.Transparent semipermeable dressings were applied over the wound. The wound area was observed on the 3rd, 7th and 10th day after trauma. The histological changes of the wound were observed by HE staining. The levels of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) were detected by radioimmunoassay. Results Compared with the model group, YYTJ significantly reduced the wound area of immune-suppressive mice (P<0.01, P<0.05) on 7 days and 10 days after treatment. The histological results showed that YYTJ promoted the proliferation of wound fibroblasts and angiogenesis. The level of IL-1β in YYTJ group was significantly higher than that in model group on 3 days (P<0.01), and there was no significant difference on 7 days, YYTJ with high/medium dose group decreased significantly on 10 days (P<0.01, P<0.05). Compared with the model group, there was no significant difference in the level of TNF-α for YYTJ groups for 3 days and the level of TNF-α significantly increased at 7 days, while the level of TNF-α in the YYTJ group decreased significantly on 10 days (P<0.01). Conclusion YYTJ can promote skin wound healing in immune-suppressive mice. The mechanism may be to repair the wounds by promoting the wound inflammatory response in the early stage, suppressing the inflammatory reaction and promoting the formation of granulation tissue in the late stage. |
| keywords:Wound healing YangYuTuJi Chronic skin ulcer |
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