| Abstract:Objective To investigate the protective effects of schisandrin B on rat model of hepatorenal syndrome and study its possible mechanism. Methods Rat model of hepatorenal syndrome was generated using common bile duct ligation, and rats were divided into five groups, including control group, model group, low-, middle-, and high-dose schisandrin B groups. The levels of ALT, TBL, CRE, UREA, quantity if urine, creatinine clearance rate, endotoxin, NO, ET and AT Ⅱ were measured. The expression levels of GRP78/94 and TLR4 mRNA in liver and kidney tissue were determined by RT-PCR. Results Schisandrin B can improve the hepatic and renal functions via inducing the levels of ALT, TBL, CRE and UREA, and suppressing quantity if urine and creatinine clearance rate in rat model of hepatorenal syndrome. Moreover, schisandrin B can stimulate the levels of GRP78 and 94 genes, and inhibit the expression of TLR4 in hepatic and renal tissue. Conclusion Schisandrin B has protected effect on rat model of hepatorenal syndrome,and the mechanism may be relevant to the expression levels of GRP78/94 and TLR4 genes. |
