| HO-1减轻大鼠颈动脉球囊损伤后的内膜增生 |
| 投稿时间：2018-02-10 修订日期：2018-03-04 点此下载全文 |
| 引用本文：杨根欢,姚荣彦,汪岩,廖鹏志,贾玉龙.HO-1减轻大鼠颈动脉球囊损伤后的内膜增生[J].医学研究杂志,2018,47(10):92-95 |
| DOI：
10.11969/j.issn.1673-548X.2018.10.025 |
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| 中文摘要:目的 探讨血红素加氧酶-1（HO-1）能否减轻大鼠颈动脉球囊损伤后的内膜增生。方法 20只雄性SD大鼠随机分为正常并注射生理盐水组、球囊损伤并注射生理盐水组，球囊损伤并注射氯化血红素组、球囊损伤并注射锌原卟啉组。建立大鼠颈动脉球囊损伤模型，14天后取各组大鼠的颈动脉。弹力蛋白染色并计算各组大鼠颈动脉的内膜面积与中膜面积的比值（i/m）。Western blot法检测各组大鼠颈动脉内HO-1、NF-κB、IL-1β、IL-6、TNF-α的表达情况。结果 球囊损伤能够明显诱导大鼠颈动脉i/m值的增加（P<0.01）。球囊损伤并注射氯化血红素组的颈动脉i/m值明显低于球囊损伤并注射生理盐水组（P<0.01）。球囊损伤并注射锌原卟啉组的颈动脉i/m值明显高于球囊损伤并注射生理盐水组（P<0.01）。球囊损伤并注射生理盐水组颈动脉的NF-κB、IL-1β、IL-6、TNF-α的表达明显高于正常并注射生理盐水组。球囊损伤并注射氯化血红素组NF-κB、IL-1β、IL-6、TNF-α的表达明显低于球囊损伤并注射生理盐水组（P<0.05）。球囊损伤并注射锌原卟啉组NF-κB、IL-1β、IL-6、TNF-α的表达明显高于球囊损伤并注射生理盐水组（P<0.01）。结论 球囊损伤能够诱导大鼠颈动脉的炎性反应及内膜增生，HO-1能够减轻大鼠颈动脉球囊损伤后的内膜增生。 |
| 中文关键词:血红素加氧酶-1 球囊损伤 炎性反应 内膜增生 |
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| Heme Oxygenase-1 can Inhibit the Intima Hyperplasia in Rat Carotid Arteries after Balloon Injury |
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| Abstract:Objective To investigate the effect of heme oxygenase-1 (HO-1) on intima hyperplasia in rat carotid arteries after balloon injury. Methods Twenty male sprague-dawley rats were randomized into groups of normal with saline injected, balloon injured with saline injected, balloon injured with hemin injected, and balloon injured with Zincprotoporphyrin injected. An established balloon-induced carotid artery injury was performed. And carotid arteries were harvested from the four groups 14 days later. The ratio of intima to media (i/m) and expression of NF-κB, IL-1β, IL-6 and TNF-α were measured. Results The hemin or Zincprotoporphyrin could induce or inhibit the expression of HO-1 in rat carotid arteries. The balloon injury could significantly induced the increasing of i/m and the expression of NF-κB, IL-1β, IL-6 and TNF-α. Hemin could minimize this effect caused by balloon injury, while Zincprotoporphyrin could aggravate this effect. Conclusion HO-1 could inhibit the intima hyperplasia in rat carotid arteries after balloon injury through alleviatting the inflammation. |
| keywords:Heme oxygenase-1 Balloon injury Inflammation Intima hyperplasia |
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