| 虾青素调控SIRT1/PGC-1α/NRF1信号通路减轻对比剂诱导的急性肾小管损伤 |
| 投稿时间：2018-01-12 修订日期：2018-03-01 点此下载全文 |
| 引用本文：徐洋,李文华,郑迪,张权.虾青素调控SIRT1/PGC-1α/NRF1信号通路减轻对比剂诱导的急性肾小管损伤[J].医学研究杂志,2018,47(11):75-79 |
| DOI：
10.11969/j.issn.1673-548X.2018.11.017 |
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| 基金项目:江苏省六大人才高峰项目（2014-YY-007） |
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| 中文摘要:目的 观察虾青素 （astaxanthin， AST） 对碘海醇 （iohexol， I） 诱导的大鼠肾小管上皮细胞损伤的保护作用，并探讨其可能机制。方法 常规培养的大鼠肾小管上皮细胞株 （NRK-52E） 分为空白对照组 （Control组）、溶剂对照组 （DMSO组）、碘海醇组 （I组）、虾青素预处理组 （AST组）、虾青素和尼克酰胺共处理组 （AST+NA组）、尼克酰胺 （nicotinamide，NA组）。CCK-8检测细胞增殖情况；硫代巴比妥酸法测定丙二醛 （MDA） 含量；流式细胞仪检测细胞内活性氧 （ROS） 的水平；Western blot法检测各组细胞SIRT1、PGC-1α和NRF1蛋白表达。结果 与Control组比较，碘海醇培养的NRK-52E细胞增殖活性明显下降，MDA和ROS水平升高，SIRT1、PGC-1α和NRF1蛋白表达明显下降 （P<0.05）。与I组比较，AST预处理组细胞增殖活性增加，MDA和ROS水平下降，SIRT1、PGC-1α和NRF1蛋白表达上调 （P<0.05）。在AST组基础上给予SIRT1抑制剂后，逆转了以上AST的保护作用。与AST+NA组比较，NA组细胞活性下降，MDA和ROS水平升高，SIRT1、PGC-1α和NRF1蛋白表达增加 （P<0.05）。结论 虾青素通过减少MDA和ROS水平，调控SIRT1/PGC-1α/NRF1信号通路缓解碘海醇诱导的NRK-52E细胞损伤。 |
| 中文关键词:对比剂急性肾损伤 SIRT1 虾青素 PGC-1α |
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| Astaxanthin Attenuates Contrast-induced Acute Kidney Injury by Regulating SIRT1/PGC-1α/NRF1 Signaling Pathway |
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| Abstract:Objective To investigate the protective effect of astaxanthin (AST) on Rat renal tubular epithelial cells (NRK-52E) injury induced by Iohexol (I), and to explore its possible mechanism. Methods NRK-52E cells were randomly devivided into six groups: normal group (Control group), vehicle group (DMSO group), iohexol group (I group), astaxanthin group (AST group), astaxanthin plus nicotinamide group (AST+NA group), and nicotinamide group (NA group). The cell proliferation was measured by CCK-8 assay. The levels of malonaldehyde (MDA) were measured by thiobarbituric acid mthod. Intracellular reactive oxygen species (ROS) levels was measured by Flow cytometry. The protein levels of SIRT1, PGC-1α and NRF1 were detected by Western Blotting. Results Compared with Control group, I group showed suppressed proliferation, the increased levels of MDA and ROS, and the expressions of SIRT1, PGC-1α and NRF1 protein were decreased. Compared to I group, AST pretreated group presented obvious proliferation, the decreased levels of MDA and ROS, and up-expressed SIRT1, PGC-1α and NRF1 protein. SIRT1 inhibitor NA administered reversed the protective effect of the AST above. Compared with the AST + NA group, NA group showed lower proliferation, the elevated levels of MDA and ROS, and the decreased expressions of SIRT1, PGC-1α and NRF1 protein, which further confirmed the protective effect of AST. Conclusion Astaxanthin could alleviate iohexol-induced NRK-52E cell injury by decreasing the levels of MDA and ROS and regulating the SIRT1/PGC-1α/NRF1 signaling pathway. |
| keywords:Contrast-induced acute kidney injury SIRT1 Astaxanthin PGC-1α |
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