| 氨甲环酸对体外人脐静脉内皮细胞损伤的保护作用 |
| 投稿时间：2018-01-01 修订日期：2018-01-02 点此下载全文 |
| 引用本文：胡磊.氨甲环酸对体外人脐静脉内皮细胞损伤的保护作用[J].医学研究杂志,2018,47(11):96-100 |
| DOI：
10.11969/j.issn.1673-548X.2018.11.022 |
| 摘要点击次数: 1184 |
| 全文下载次数: 1076 |
|
| 基金项目:浙江省重大科技专项基金资助项目（2016C03021） |
|
| 中文摘要:目的 研究氨甲环酸（tranexamic acid， TXA）对H2O2诱导人脐静脉内皮细胞（human umbilical vein endothelial cells， HUVECs）损伤的保护作用及给药时机选择。方法 体外培养HUVECs，加入100μmol/L的H2O2作用12h，在H2O2作用开始后30min和60min以及H2O2作用结束前1h和2h这4个时间点加入TXA （150μmol/L）；Hoechst 33258染色法观察HUVECs形态；Western blot法检测细胞内细胞间黏附分子（intercellular cell adhesion molecule， ICAM）的表达；酶联免疫吸附双抗体夹心法（ELISA） 检测细胞上清液多配体聚糖、tPA和PAI-1含量。结果 HUVECs经H2O2作用后，可显著诱导细胞凋亡，上调ICAM、多配体聚糖和tPA的表达，同时抑制PAI-1的表达；TXA在H2O2作用60min内给药可抑制H2O2对HUVECs的凋亡诱导作用，同时下调ICAM、多配体聚糖和tPA的表达，并上调PAI-1的表达，TXA在60min后给药无上述保护作用。结论 早期TXA给药对H2O2诱导HUVECs损伤有明显抗纤维蛋白溶解和保护作用，从而表明临床应用TXA需早期给药。 |
| 中文关键词:HUVEC TXA 内皮性损伤 |
| |
| Tranexamic Acid Ameliorates the Injury of Human Umbilical Vein Endothelial Cells in Vitro. |
|
|
| Abstract:Objective To investigate the protective effect of tranexamic acid (TXA) on human umbilical vein endothelial cells (HUVECs) following H2O2 injury. Methods In vitro HUVECs were exposed to H2O2 to stimulate post-shock reperfusion. TXA was added during H2O2 incubation (30 minutes and 60 minutes) and at 1 hour and 2 hours before the termination of H2O2 exposure. The cellular morphology was observed using a fluorescence microscope after Hoechst 33258 staining. Endothelial cell injury was indexed by ICAM expression, syndecan release (marker for glycocalyx injury), tissue type plasminogen activator (tPA) and plasminogen activator inhibitor-1 (PAI-1). Results Endothelial activation and injury as indexed by apoptosis and ICAM expression were increased by H2O2 incubation. Biomarkers of endothelial coagulation profile (tPA/PAI-1) demonstrated a profibrinolytic profile (increased tPA and tPA/PAI-1 ratio) after H2O2 treatment. TXA administration within 60 minutes of H2O2 treatment abolished the adverse effects of HUVECs induced by H2O2. TXA administration after 60 minutes was not protective effect on HUVECs. Conclusion Antifibrinolytic and other protective effects of TXA administration on endothelial injury are time-dependent. This study supports the concept that the clinical efficacy of TXA administration requires "early administration". |
| keywords:HUVEC Tranexamic acid Endothelial injury |
| 查看全文 查看/发表评论 下载PDF阅读器 |
