| 和厚朴酚通过上调miR-99a/b抑制HeLa细胞增殖和侵袭 |
| 投稿时间：2018-01-13 修订日期：2018-01-25 点此下载全文 |
| 引用本文：徐雅杰,张志强,于鹤.和厚朴酚通过上调miR-99a/b抑制HeLa细胞增殖和侵袭[J].医学研究杂志,2018,47(11):101-106 |
| DOI：
10.11969/j.issn.1673-548X.2018.11.023 |
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| 基金项目:河南省医学科技攻关计划项目（201603094） |
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| 中文摘要:目的 探究和厚朴酚对宫颈癌HeLa细胞增殖和侵袭的影响，并初步探讨相关分子机制。方法 将培养的对数期的人宫颈癌HeLa细胞分为对照组、Scramble组、miR-99a/b mimic组、和厚朴酚高、中、低剂量组。MTT实验用于检测不同干预对HeLa细胞增殖的影响，Transwell小室用于测定HeLa的侵袭能力，实时荧光定量PCR（qRT-PCR）用于检测细胞中miR-99a、miR-99b的表达情况，蛋白免疫印迹（WB）检测细胞中 cyclin D1、MMP7蛋白表达。结果 和厚朴酚高、中、低剂量组细胞增殖率和侵袭细胞数均明显低于对照组和DMSO溶剂组（P<0.05），并呈剂量依赖性。minic组和厚朴酚高、中、低剂量组miR-99a和miR-99b表达水平明显高于对照组和Scramble组（P<0.05）；随着和厚朴酚剂量的增加，HeLa细胞中miR-99a和miR-99b表达水平逐渐升高。和厚朴酚高、中、低剂量组miR-99a和miR-99b表达水平明显高于对照组和Scramble组（P<0.05），且呈剂量依赖性。和厚朴酚高、中、低剂量组HeLa细胞增殖率和侵袭能力明显低于对照组和Scramble组，呈现剂量依赖性。Western blot法检测结果显示，minic组和和厚朴酚高、中、低剂量组cyclin D1和MMP7表达水平明显低于对照组和Scramble组（P<0.05）；随着和厚朴酚剂量的增加，HeLa细胞中cyclin D1和MMP7表达水平明显降低。结论 和厚朴酚可能通过上调miR-99a/b表达，进而抑制宫颈癌HeLa细胞增殖和侵袭，为宫颈癌的靶向治疗提供一定的理论依据。 |
| 中文关键词:和厚朴酚 宫颈癌 miR-99a miR-99b |
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| Honokiol Inhibits HeLa Cell Proliferation and Invasion by Up-regulating miR-99a/b |
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| Abstract:Objective To explore the influence of Honokiol on proliferation and invasion of hela cells with the related molecular mechanisms. Methods MTT assay was used to detect the effects of different interventions on the proliferation of HeLa cells. Transwell chamber was performed to determine the invasion ability of Hela cells. Real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-99a and miR-99b. Western blot (WB) was used to detect the protein level of cyclin D1, MMP7. Results The proliferation and invasive cell numbers of honokiol in high, medium and low dose groups were significantly lower than those in control group and DMSO solvent group (P<0.05), and in a dose-dependent manner. The levels of miR-99a and miR-99b in minic group and honokiol group were significantly higher than those in control group and Scramble group (P<0.05). With the increase of honokiol dose, miR -99a and miR-99b expression levels gradually increased. The expression levels of miR-99a and miR-99b in high, medium and low doses of honokiol were significantly higher than those in the control and Scramble groups (P<0.05), and were dose-dependent.MiR-99a and miR-99b expression levels in HeLa cells of honokiol mid-dose group and honokiol high-dose group were significantly lower than those in the miR-99a/b mimic groups (P<0.05).Western blot results showed that the expressions of cyclin D1 and MMP7 in minic group and honokiol group were significantly lower than those in control group and Scramble group (P<0.05). With the increase of honokiol dose, HeLa Cell cyclin D1 and MMP7 expression levels were significantly lower. Conclusion Honokiol may inhibit the proliferation and invasion of cervical cancer Hela cells by up-regulating the expression of miR-99a/b, which will provide a theoretical basis for the diagnosis and targeted therapy of cervical cancer. |
| keywords:Honokiol cervical cancer MiR-99a MiR-99b |
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