树突状细胞、Treg细胞在SLE伴血小板计数减少患者外周血中的表达
投稿时间:2018-01-21  修订日期:2018-03-28  点此下载全文
引用本文:王向丽,沈育娟,吴艳群,李光文,邢倩.树突状细胞、Treg细胞在SLE伴血小板计数减少患者外周血中的表达[J].医学研究杂志,2018,47(11):132-136
DOI: 10.11969/j.issn.1673-548X.2018.11.029
摘要点击次数: 638
全文下载次数: 582
作者单位E-mail
王向丽 266071 青岛大学医学院附属青岛市市立医院  
沈育娟 266071 青岛大学医学院附属青岛市市立医院  
吴艳群 266071 青岛大学医学院附属青岛市市立医院  
李光文 266071 青岛大学医学院附属青岛市市立医院  
邢倩 266071 青岛大学医学院附属青岛市市立医院 gdz696@163.com 
基金项目:山东省青岛市科技局基金资助项目(13-1-3-19-nsh);山东省青岛市医疗卫生重点学科建设项目
中文摘要:目的 探讨树突状细胞(DC)及CD4+CD25+FoxP3+调节性T细胞(Treg)在系统性红斑狼疮(SLE)伴中重度血小板计数减少患者外周血中的表达及临床意义。方法 选取SLE伴中、重度血小板计数减少患者32例,SLE伴血小板计数正常患者28例,正常健康人30例,应用流式细胞术检测各组外周血中髓系DC(mDC)、浆细胞样DC(pDC)治疗前后比率变化及与Treg细胞的相关性,酶联免疫吸附法(ELISA法)检测外周血IL-12、IFN-α、TGF-β水平。结果 SLE伴血小板计数减少组外周血pDC比率显著低于SLE伴血小板计数正常组患者(P<0.05)。SLE伴血小板计数减少组激素治疗后pDC比率较治疗前减少,Treg细胞比率较治疗前增加,pDC与Treg细胞呈负相关(r=-0.537, P<0.05)。SLE伴血小板计数减少组患者外周血DC表面协同刺激分子CD86表达的MFI明显高于SLE伴血小板计数正常组及健康对照组。SLE患者外周血IL-12、IFN-α水平与健康对照组比较显著升高,TGF-β水平显著降低(P<0.05)。结论 pDC可能参与了SLE伴中重度血小板计数减少的免疫发病机制,并与Treg细胞有一定的相关性。
中文关键词:系统性红斑狼疮  血小板计数减少  树突状细胞  Treg细胞
 
Significance of Peripheral Dendritic Cells and Treg cells in Patients with Systemic Lupus Erythematosus Associated with Thrombocytopenia
Abstract:Objective To explore the expression and significance of circulating dendritic cells (DC) and regulatory T cells (Treg) in patients with systemic lupus erythematosus (SLE) with moderate or severe thrombocytopenia. Methods Totally 60 patients with SLE were selected, including 32 patients with moderate or severe thrombocytopenia, 28 patients with normal platelets, and 30 healthy controls. Flow cytometry was used to analyze the proportion of peripheral blood myeloid DC (mDC), plasmacytoid DC (pDC) and correlation to CD4+CD25+FoxP3+ Treg cells before and after the treatment. Results The proportion of pDC in peripheral blood of SLE with moderate or severe thrombocytopenia was significantly decreased than that in SLE patients with normal platelets (P<0.05). After corticosteroid treatment, the proportion of pDC was decreased than that before treatment, while the proportion of Treg cells was increased,there was inverse correlation between pDC and Treg cells after treatment (r=-0.537,P<0.05). The serum levels of IL-12 and IFN-α in SLE were significantly higher while the serum level of TGF-β was significantly decreased than that in healthy controls. Conclusion pDCs may play a role in the pathogenesis of SLE with thrombocytopenia, and have a certain correlation with Treg cells.
keywords:Systemic lupus erythematosus  Thrombocytopenia  Dendritic cells  Treg cells
查看全文  查看/发表评论  下载PDF阅读器

京公网安备 11010502037822号