虫草素对人骨肉瘤MG-63细胞凋亡的影响及其机制研究
投稿时间:2017-12-28  修订日期:2018-03-16  点此下载全文
引用本文:梅红军,刘洋.虫草素对人骨肉瘤MG-63细胞凋亡的影响及其机制研究[J].医学研究杂志,2018,47(12):75-78
DOI: 10.11969/j.issn.1673-548X.2018.12.018
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作者单位E-mail
梅红军 430050 武汉市第五医院骨科 dreamingmhj@163.com 
刘洋 430050 武汉市第五医院骨科  
基金项目:国家自然科学基金资助项目(81341078);武汉市科技计划项目(2014062801011264)
中文摘要:目的 研究虫草素对人骨肉瘤MG-63细胞凋亡的影响及其机制。方法 采用CCK-8实验检测不同浓度虫草素作用于MG-63骨肉瘤细胞24h和48h后对癌细胞增殖的抑制作用;采用流式细胞术分析虫草素对MG-63细胞周期分布及凋亡的影响;采用Western blot法检测虫草素对MG-63细胞凋亡相关蛋白Bax、Bcl-2、cleaved caspase-9、cleaved caspase-3及NICD1、Hes1蛋白表达的影响。结果 虫草素对人骨肉瘤MG-63细胞有明显的增殖抑制作用,导致MG-63细胞周期阻滞于G0/G1期并诱导细胞凋亡;虫草素可上调Bax、cleaved caspase-9和cleaved caspase-3蛋白的表达并下调Bcl-2,NICD1和Hes1蛋白的表达。结论 虫草素通过抑制骨肉瘤细胞增殖,诱导细胞周期阻滞于G0/G1期并通过线粒体凋亡途径诱导MG-63细胞凋亡从而发挥抗骨肉瘤作用,可能骨肉瘤细胞中Notch信号通路活性下调有关。
中文关键词:虫草素  MG-63细胞  凋亡  Notch信号通路
 
Effect of Cordcepin on Apoptosis of MG-63 Osteoarcoma Cells and its Mechanism
Abstract:Objective To study the effect of cordcepin on proliferation and apoptosis of MG-63 osteosarcoma cells and explore its mechanism. Methods MG-63 cells were treated with vary concentrations of cordcepin, the proliferation of MG-63 cells were detected by CCK-8 assay, the cell cycle distribution and apoptosis rate of MG-63 cells were determined by flow cytometry. The expression levels of Bax, Bcl-2, cleaved caspase-9, cleaved caspase-3 NICD1 and Hes1 were detected by western blot. Results CCK-8 assay showed that cordcepin inhibits proliferation of MG-63 cells in a concentration-and time-dependent manner. The results of flow cytometry suggested that cordcepin treatment could arrest cell cycle at G0/G1 phase and induce apoptosis of MG-63 cells. The expression levels of Bax, cleaved caspase-9 and cleaved caspase-3 increased with the treatment of cordcepin, whereas the protein levels of Bcl-2, NICD1 and Hes1 decreased. Conclusion Cordcepin exerts anti-osteosarcoma effects through inhibiting the proliferation, inducing cell cycle arrest and apoptosis, which is related with the down-regulation of Notch signaling pathway.
keywords:Cordcepin  MG-63 cell  Apoptosis  Notch signaling pathway
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