| 雌激素缺乏对心肌衰老、凋亡和心功能的影响 |
| 投稿时间：2018-01-06 修订日期：2018-01-19 点此下载全文 |
| 引用本文：严翼,张浩,杨志健,苗登顺,张定国.雌激素缺乏对心肌衰老、凋亡和心功能的影响[J].医学研究杂志,2018,47(12):110-114 |
| DOI：
10.11969/j.issn.1673-548X.2018.12.026 |
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| 中文摘要:目的 通过手术去除双侧卵巢建立更年期小鼠模型探讨雌激素缺乏对心脏衰老、凋亡和心功能的影响，并讨论相关机制。方法 取8周龄雌性C57/BL6小鼠20只，按随机数字表法分为去卵巢组（OVX group）和假手术对照组（control group）（每组各10只）。去卵巢组通过手术去除双侧卵巢建立更年期模型。术后6个月行心脏超声（以下简称心超）检测评估心功能，心肌组织取材进行组织学检测和衰老、凋亡等相关指标检测。结果 与对照组比较，OVX组小鼠心肌纤维化和心/体重比均明显升高。二维心超结果显示OVX组小鼠较对照组小鼠室间隔厚度、舒张期左心室内径和容量显著增高。与对照组比较，OVX组小鼠β-gal阳性细胞率明显增加，P16、P19表达显著上调，而SIRT1、Bmi-1等抗衰老指标显著降低。与对照组比较，OVX组小鼠凋亡指标Bax、caspase-3等表达显著增加，抗凋亡指标Bcl-2表达明显降低。与对照组比较，OVX组小鼠抗氧化指标SOD1、SOD2表达均显著下降。结论 雌激素缺乏能够促进心肌纤维化、导致心肌细胞衰老和凋亡，其主要机制可能与降低SIRT1和Bmi-1的表达促进氧化应激等多种因素相关。 |
| 中文关键词:雌激素 心脏 衰老 凋亡 |
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| Research of Estrogen Deficiency on Myocardial Aging, Apoptosis and Cardiac Function |
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| Abstract:Objective To investigate the effects and mechanism of estrogen on heart aging, apoptosis and cardiac function by establishing the menopause model through removing bilateral ovaries. Methods A total of 20 eight-weeks-old female C57/BL6 mice were randomly divided into ovariectomized group (OVX group) and sham operated group (control group) (n=10). Bilateral ovaries were removed to establish the menopause model. Six months later, cardiac function was assessed by echocardiography, and the myocardial tissue was collected to testing the aging and apoptosis indicators. Results Compared with the control group, OVX group showed a definite increase in myocardial fibrosis and heart/body weight ratio. Echocardiography showed that IVS, EDV and LVIDd were higher than those in the control group. In OVX group, the beta-gal positive cell rate and the expression of P16, P19 were increased significantly, while SIRT1, Bmi-1 and other anti-aging indicators reduced profoundly, when compared with those in control group. At the same time, the expression of Bax, caspase-3 in OVX group increased significantly, whereas the expression of anti-apoptotic index Bcl-2 reduced markedly, when compared with those in control group. Furthermore, the expression of the antioxidant index SOD1 and SOD2 in OVX group decreased notably when compared with control group. Conclusion Lack of estrogen can promote ventricular wall hypertrophy, increase myocardial fibrosis, accelerate the cell aging and apoptosis, which may be related to decreasing the expression of SIRT1 and Bmi-1, promoting the oxidative stress. |
| keywords:Estrogen Heart Aging Apoptosis |
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