Ki-67在下咽鳞状细胞癌中的表达及其与放射敏感度的关系
投稿时间:2017-09-04  修订日期:2017-09-19  点此下载全文
引用本文:黄春梅,李嵘,罗娟.Ki-67在下咽鳞状细胞癌中的表达及其与放射敏感度的关系[J].医学研究杂志,2018,47(12):115-119,176
DOI: 10.11969/j.issn.1673-548X.2018.12.027
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作者单位E-mail
黄春梅 620000 眉山市人民医院肿瘤科 huangchunmei1983@126.com 
李嵘 620000 眉山市人民医院肿瘤科  
罗娟 620000 眉山市人民医院肿瘤科  
基金项目:四川省卫生厅科研基金资助项目(140039)
中文摘要:目的 探究Ki-67在下咽鳞状细胞癌组织中的表达情况及其与下咽鳞癌患者放射敏感度的关系。方法 收集180例放疗前经活检诊断为下咽鳞状细胞癌的肿瘤组织标本,另取20例正常下咽黏膜组织标本,采用免疫组化方法检测组织标本中Ki-67蛋白表达情况。采用6MV-X线直线加速器对下咽鳞癌患者进行常规放射治疗,放疗结束后根据X线片评价标准评估疗效。分析Ki-67表达与患者临床病理特征及单纯放疗疗效的关系。采用Kaplan-Meier法评估Ki-67表达与患者放疗后生存率间的关系。结果 Ki-67在下咽鳞癌组织中的蛋白表达强度(97.7%)显著高于正常下咽黏膜组织(0.0%),差异有统计学意义(P<0.05);Ki-67表达与下咽鳞癌患者临床分期、淋巴结转移有关(P<0.05),与患者性别、年龄、原发肿瘤直径及组织分化程度等差异均无统计学意义(P>0.05);Ki-67表达强度与近期放疗疗效呈正相关(P<0.05);Ki-67表达强度与远期PFS和OS均有关(P>0.05)。多元Logistic回归分析表明临床分期、淋巴结转移、Ki-67表达强度与放疗疗效相关。结论 Ki-67的表达能反映出下咽鳞状细胞癌放射敏感度,可作为预测下咽鳞癌放疗效果的重要指标。
中文关键词:Ki-67  下咽部  鳞状细胞癌  放射敏感度  免疫组化
 
Ki-67 Expression in Hypopharyngeal Squamous Cell Carcinoma and Its Relationship with Radiosensitivity
Abstract:Objective To explore Ki-67 expression in hypopharyngeal squamous cell carcinoma and its relationship with radiosensitivity of patient with hypopharyngeal squamous cell carcinoma. Methods Totally 180 cases of tumor tissue specimen of hypopharyngeal squamous cell carcinoma diagnosed by biopsy before radiotherapy, and another 20 cases of normal hypopharyngeal mucosa tissue specimens were selected. The expression of Ki-67 protein in tissue specimens was detected by using SP immunohistochemical method, and routine radiotherapy was on patients with hypopharyngeal squamous cell carcinoma by using 6MV-X ray linac, and after radiotherapy, curative effect was evaluated by X ray evaluation criteria. The relationship between Ki-67 expression and clinical pathological features and radiotherapy alone was analyzed. Relationship between Ki-67 expression and survival rate after radiotherapy was evaluated by using Kaplan-Meier method. Results Ki-67 protein expression intensity in hypopharyngeal squamous cell carcinoma (97.7%) was significantly higher than that (0.0%) in normal hypopharyngeal mucosa, with statistically significant difference (P<0.05). Ki-67 expression was related to clinical stage and lymph node metastasis of hypopharyngeal squamous cell carcinoma (P<0.05). There was no significant correlation between Ki-67 expression and sex, age, tumor size, and tumor differentiation (P>0.05). The expression intensity of Ki-67 was positively correlated with short-term radiotherapy efficacy (P<0.05). The expression intensity of Ki-67 was also positively correlated with long-term PFS and OS (P<0.05). Multivariate Logistic regression analysis showed that clinical stage, lymph node metastasis and the expression intensity of Ki-67 were related to the radiotherapy efficacy. Conclusion Ki-67 expression can reflect radiosensitivity of hypopharyngeal squamous cell carcinoma. It can be used as an important index to predict the effect of radiotherapy for hypopharyngeal squamous cell carcinoma.
keywords:Ki-67  Hypopharynx  Squamous cell carcinoma  Radiosensitivity  Immunohistochemistry
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