| 人参皂苷Rg3通过下调PD-L1逆转胃癌细胞的顺铂耐药性 |
| 投稿时间：2018-02-05 修订日期：2018-04-04 点此下载全文 |
| 引用本文：万品文,王倩,万春.人参皂苷Rg3通过下调PD-L1逆转胃癌细胞的顺铂耐药性[J].医学研究杂志,2018,47(12):120-124 |
| DOI：
10.11969/j.issn.1673-548X.2018.12.028 |
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| 基金项目:河南省医学科技攻关计划项目（201405033） |
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| 中文摘要:目的 探究人参皂苷Rg3对顺铂（DDP）耐药性胃癌细胞株SGC7901/DDP的逆转作用及机制。方法 CKK-8法鉴定Rg3对SGC7901/DDP细胞的毒性，筛选出合适浓度进行实验；将SGC7901/DDP细胞分为5组，空白对照组、Rg3低剂量组（10μg/ml）、中剂量组（20μg/ml）、高剂量组（40μg/ml）、维拉帕米组（10μg/ml），CCK-8法检测Rg3对SGC7901/DDP细胞顺铂耐药性的逆转作用；实时定量PCR（qRT-PCR）和蛋白免疫印记（WB）检测PD-L1mRNA和蛋白表达；CCK-8法检测SGC7901/DDP细胞PD-L1过表达后，Rg3对细胞耐药性的作用。结果 5、10、20、40、80、160μg/ml Rg3均能抑制SGC7901、SGC7901/DDP细胞活性，呈剂量和时间依赖效应，选择5、10、20、40μg/ml作为人参皂苷Rg3实验浓度。与DPP组比较，Rg3处理后DDP对SGC7901/DDP细胞增殖的抑制作用明显增强（P<0.05）。与SGC7901细胞比较，SGC7901/DDP细胞中PD-L1蛋白显著高表达（P<0.05）；与DPP组比较，Rg3处理后细胞中PD-L1mRNA及蛋白表达水平均显著降低（P<0.05）。SGC7901/DDP细胞PD-L1过表达可有效减弱Rg3对SGC7901/DDP细胞耐药性的逆转作用。结论 人参皂苷Rg3可有效抑制SGC7901/DDP细胞增殖，增加SGC7901/DDP对顺铂的敏感度，其机制可能通过下调PD-L1基因表达实现。 |
| 中文关键词:人参皂苷Rg3 顺铂 PD-L1 胃癌细胞 耐药性 |
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| Ginsenoside Rg3 Reverses Cisplatin Resistance of Gastric Cancer Cells by Down-Regulation of PD-L1 |
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| Abstract:Objective To investigate the reversal effect of Ginsenoside Rg3 on Cisplatin (DDP) resistant gastric cancer cell line SGC7901/DDP and its mechanism. Methods The CKK-8 method was used to identify the toxicity of Rg3 to SGC7901/DDP cells and to screen out the appropriate concentration for the experiment; SGC7901/DDP cells were divided into 5 groups, control group, low dose Rg3 group (10g/ml), middle dose group (20g/ml), high dose group (40g/ml), Verapamil group (10g/ml), CCK-8 assay was used to detect the reversal effect of Rg3 on Cisplatin resistance in SGC7901/DDP cells; real-time quantitative PCR (qRT-PCR) and Western blot (WB) were used to detect the expressions of PD-L1mRNA and protein; CCK-8 assay was used to detect the effect of Rg3 on the drug resistance after PD-L1 overexpression in the SGC7901/DDP cells. Results 5, 10, 20, 40, 80, 160μg/ml Rg3 could inhibit SGC7901 and SGC7901/DDP cell activities, in dose and time dependences. Choosed 5, 10, 20, 40μg/ml as the experimental concentrations of Ginsenoside Rg3 Compared with the DPP group, the inhibitory effect of DDP after Rg3 treatment on the proliferation of SGC7901/DDP cells was significantly enhanced (P<0.05). Compared with SGC7901 cells, the expression of PD-L1 protein in SGC7901/DDP cells was high (P<0.05); compared with the DPP group, the expression levels of PD-L1mRNA and protein in the cells decreased significantly after Rg3 treatment (P<0.05). The overexpression of PD-L1 in SGC7901/DDP cells can effectively reduce the reversal effect of Rg3 on the drug resistance of SGC7901/DDP cells. Conclusion Ginsenoside Rg3 can effectively inhibit the proliferation of SGC7901/DDP cells and increase the sensitivity of SGC7901/DDP to Cisplatin, and its mechanism may be achieved by down-regulating the expression of PD-L1 gene. |
| keywords:Ginsenoside Rg3 Cisplatin PD-L1 Gastric cancer cells Drug resistance |
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