| 脊髓背根神经节ZXDC介导CCL2对慢性压迫性神经损伤小鼠神经性疼痛的作用 |
| 投稿时间：2022-10-22 修订日期：2022-11-30 点此下载全文 |
| 引用本文：李文媛,王晓宇,吕忠孝,刘东明,李艺,王淑影,王莹.脊髓背根神经节ZXDC介导CCL2对慢性压迫性神经损伤小鼠神经性疼痛的作用[J].医学研究杂志,2024,53(1):56-62 |
| DOI：
10.11969/j.issn.1673-548X.2024.01.013 |
| 摘要点击次数: 92 |
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| 基金项目:国家自然科学基金资助项目(81870977)；黑龙江省自然科学基金资助项目(JQ2021H004)；黑龙江省属高校基本科研业务费项目(2021-KYYWF-0469)；牡丹江医学院科学基金火炬计划项目(2022-MYHJ-012)；牡丹江医学院研究生导师科研专项计划项目(YJSZX2022007) |
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| 中文摘要:目的 探讨脊髓背根神经节(dorsal root ganglion, DRG)中转录因子锌指X连锁复制C(zinc finger X-linked duplicated C,ZXDC)介导CCL2/CCR2信号通路对慢性压迫性神经损伤(chronic construction injury model, CCI)诱导神经性疼痛的作用及机制。方法 构建小鼠坐骨神经慢性压迫性损伤模型,免疫荧光染色、Western blot法和实时荧光定量PCR(RT-qPCR)检测正常情况和CCI造模后DRG中ZXDC及CCL2表达变化；将实验动物分为假手术(Sham)组、CCI+AAV-NC组和CCI+AAV-ZXDC siRNA组；Western blot法和免疫荧光染色检测各组小鼠CCI造模后各时间点DRG中ZXDC、CCL2和CCR2表达,RT-qPCR检测DRG中促炎性细胞因子TNF-α和IL-1β mRNA表达,机械性缩足反射测试检测神经性疼痛行为改变。结果 ZXDC表达定位在DRG大、中、小神经元。CCI损伤后1~3天 DRG中ZXDC和CCL2 蛋白和mRNA表达显著增高,CCI后7天二者表达显著降低,ZXDC和CCL2mRNA表达量呈正相关(P均<0.05)。CCI后3天,与Sham组比较,CCI+AAV-NC组和CCI+AAV-ZXDC siRNA组ZXDC、CCL2、CCR2蛋白表达、TNF-α和IL-1β mRNA表达显著增高,其中CCI+AAV-ZXDC siRNA组较CCI+AAV-NC组ZXDC、CCL2、CCR2蛋白表达、TNF-α和IL-1β mRNA显著降低(P均<0.05)。与Sham组比较,CCI+AAV-ZXDC siRNA组和CCI+AAV-NC组CCI后各时间点机械缩足阈值均显著降低,其中CCI后7天,CCI+AAV-ZXDC siRNA组机械缩足阈值显著高于CCI+AAV-NC组(P均<0.05)。结论 脊髓背根神经节ZXDC基因敲减通过抑制CCL2/CCR2信号通路介导CCI诱导的神经性疼痛。 |
| 中文关键词:脊髓背根神经节 锌指X连锁复制C CC趋化因子2 神经损伤 慢性神经疼痛 |
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| Construction and Application of the Big Data Research Platform for Biological Sample Information Resources in a Large Comprehensive Hospital |
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| Abstract:Purpose/Significance By integrating clinical and biological sample information, a big data research platform for biological sample information resources is built to provide one-stop data retrieval, integration and analysis services for researchers, and a data governance system is established, so as to improve the level of hospital clinical research infrastructure construction.Method/Process Common data model and data governance technology are adopted to integrate data sources from different vendors through extraction, transformation, loading and other steps to provide a unified data access portal. Result/Conclusion The big data research platform for biological sample information resources has the advantages of multi-dimensional data screening and rapid integrated analysis, which can provide support for clinical research. |
| keywords:biospecimen medical big data data governance common data model |
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