| miRNA-449a在肺癌组织中的表达及其检测意义 |
| 投稿时间：2014-06-12 修订日期：2014-06-25 点此下载全文 |
| 引用本文：林剑勇,邓益斌,陆小婵.miRNA-449a在肺癌组织中的表达及其检测意义[J].医学研究杂志,2015,44(1):63-65 |
| DOI：
10.3969/j.issn.1673-548X.2015.01.018 |
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| 基金项目:广西壮族自治区自然科学基金资助项目(2011GXNSFA04215) |
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| 中文摘要:目的 探讨miRNA-449a在肺癌组织中的表达及其临床应用价值。方法 收集右江族医学院附属医院2011年1月1日~2013年6月30日收治的58例肺癌患者为研究对象,采用反转录荧光定量聚合酶链式反应(RT-PCR)检测miRNA-449a在肺癌组织和癌旁正常组织中的表达量,分析miRNA-449a与临床病理特征的关系,并采用荧光电子显微镜观察miRNA-449a模拟物对体外培养肺癌细胞株95D细胞凋亡的影响。结果 鳞癌组和腺癌组miRNA-449a平均表达量均明显低于癌旁正常对照组(LSD-t=6.712,P=0.000; LSD-t=4.572,P=0.000),其相对表达量与瘤体大小(u=78.412,P=0.012)有关,与患者年龄(u=920.000,P=0.615)、性别(u=800.000,P=0.215)、病理类型(χ2=4.221,P=0.155)和临床分期(u=754.000,P=0.009)无关。与阴性对照组比较,miRNA-449a模拟物可明显促进肺癌细胞的凋亡,且呈剂量依赖性。结论 miRNA-449a在肺癌组织中呈低表达,可诱导肺癌细胞凋亡,发挥抑癌基因的作用,可能成为肺癌早期诊断与治疗的新分子靶标。 |
| 中文关键词:miRNA-449a 肺癌 miRNAs 分子诊断 |
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| Expression and Biological Function of miRNA-449a in Lung Cancer |
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| Abstract:Objective To investigate the expression and biological function of miRNA-449a in lung cancer. Methods A total of 58 patients with lung cancer were selected as research objects. The expression of miRNA-449a in lung cancer tissues and matched normal tissues were detected by real time PCR. And the relationship between age, sex, tumor size and the level of miRNA-449a were analysed. Chemiluminescence assay was applied to analyze the apoptosis after transfecting mimics of miRNA-449a into non-small cell lung cancer. Results The level of miRNA-449a was significantly lower in squamous carcinoma group and adenocarcinoma group than that of control group(LSD-t=6.712,P=0.000; LSD-t=4.572,P=0.000; respectively). There was correlation between miRNA-449a and tumor size(u=78.412,P=0.012) and no correlation between miRNA-449a and age(u=920.000,P=0.615), gender(u=800.000,P=0.215), pathological type(χ2=4.221,P=0.155) and clinic stage(u=754.000,P=0.009), all the statistic P were higher than 0.05. Compared with negative control, miRNA-449a mimics could induce apoptosis of lung cancer cell 95D with dose-effect dependent. Conclusion miRNA-449a was down-regulated expression in lung cancer and induced apoptosis, which maybe play a role as a tumor suppressor gene. |
| keywords:miRNA-449a Lung cancer MicroRNA Molecular diagnostic |
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