| 双氯芬酸钠对大鼠骨髓间充质干细胞影响的浓度、时间效应 |
| 投稿时间：2014-08-01 修订日期：2014-08-29 点此下载全文 |
| 引用本文：徐伟,洪盾,陈真,袁赤亭,陈海啸.双氯芬酸钠对大鼠骨髓间充质干细胞影响的浓度、时间效应[J].医学研究杂志,2015,44(2):46-49 |
| DOI：
10.3969/j.issn.1673-548X.2015.02.013 |
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| 基金项目:国家自然科学基金资助项目(81171748) |
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| 中文摘要:目的 通过不同浓度的双氯芬酸钠干预体外培养的大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMSCs), 观察其对大鼠骨髓间充质干细胞增殖、凋亡及成骨分化能力的影响;不同药物干预时间对细胞成骨分化能力的影响。方法 不同浓度的双氯芬酸钠(6.4、3.2、1.6、0.8、0.4mg/L)干预第4代(P4)BMSCs, 通过MTS法检测细胞增殖活性; Annexin Ⅴ-FITC法检测细胞凋亡;根据茜素红染色钙结节的数量, 初步检测细胞成骨能力。并选取1.6mg/L的药物浓度, 不同时间点和时长进行干预, 检测细胞成骨分化能力的变化。结果 随着双氯芬酸钠浓度的升高, BMSCs细胞增殖活性逐渐降低, 细胞凋亡比率升高, 钙结节数量减少。药物在第1~14天期间干预能够减少钙结节的形成, 在第14~21天期间干预对钙结节的形成无明显影响。结论 双氯芬酸钠可影响BMSCs的增殖、凋亡及成骨分化能力, 且呈浓度和时间依赖性, 短期的作用效果是部分可逆的。 |
| 中文关键词:双氯芬酸钠 骨髓间充质干细胞 浓度效应 时间效应 |
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| Concentration Effect and Time Effect of Diclofenac Sodium on Bone Marrow Mesenchymal Stem Cells |
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| Abstract:Objective To research the impact of different concentrations of Diclofenac sodium on the biological characteristics of rat bone marrow mesenchymal stem cells(Bone marrow mesenchymal stem cells, BMSCs)morphology, proliferation, apoptosis, osteogenic differentiation, and the impact of different intervention time on osteogenic differentiation of cells. Methods Different concentrations of Diclofenac sodium (6.4, 3.2, 1.6, 0.8, 0.4mg/L)intervented the fourth-generation (P4) of BMSCs.BMSCs proliferation was detected by MTS assay. Apoptosis was detected by Annexin Ⅴ-FITC assay.Osteogenic differentiation capacity changes detected. We select the 1.6mg/L drug concentration, different time point and time to intervene, and detect the osteogenic differentiation. Results With the increase of diclofenac sodium concentration, cell proliferation activity of BMSCs decreased, the cell apoptosis rate increased, the number of calcium nodules reduced. The drug can reduce the formation of calcium nodules in 1-14 days during the intervention. In the 14-21 days during the intervention on the formation of calcium nodules had no obvious effect. Conclusion Diclofenac sodium can affect BMSCs proliferation, apoptosis and osteogenic differentiation capacity, with a concentration and time dependent manner. The short-term effect is partially reversible. |
| keywords:Diclofenac sodium BMSCs Concentration effect Time effect |
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