| proBDNF-p75NTR通路抑制C6细胞增殖 |
| 投稿时间：2014-07-21 修订日期：2014-09-17 点此下载全文 |
| 引用本文：熊静,李志伟,韩剑虹,殷梅,李馨蕊,朱榆红.proBDNF-p75NTR通路抑制C6细胞增殖[J].医学研究杂志,2015,44(3):89-93 |
| DOI：
10.3969/j.issn.1673-548X.2015.03.025 |
| 摘要点击次数: 1979 |
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| 基金项目:云南省卫生厅内设机构基金资助项目(2012WS0080) |
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| 中文摘要:目的 proBDNF和成熟BDNF分别通过p75NTR受体和TrkB受体介导相反的生物学作用。微环境变化时, proBDNF-p75信号通路和成熟BDNF-TrkB通路不平衡可能对细胞生长和增殖产生不同影响。本研究拟探讨不同培养条件下, C6细胞表达proBDNF及其受体p75NTR的变化, 对C6细胞生长和增殖的作用。方法 用细胞免疫荧光双标和Western blot方法, 检测在含不同浓度血清培养液中培养的C6细胞proBDNF及其受体p75NTR表达。分别用特异性的proBDNF 抗体阻断内源性proBDNF, 用p75NTR-ECD-Fc 阻断p75NTR, 用MTT法和Brdu法分别测定内源性proBDNF及其受体p75NTR对C6细胞生长和增殖的作用。结果 proBDNF和p75NTR在无血清培养的C6细胞中共表达明显上调, 加入proBDNF抗体(10μg/ml)处理细胞后, C6细胞的生长和增殖明显增加。p75NTR的作用取决于其在不同环境下结合的配体。结论 proBDNF-p75NTR通路起到平衡成熟BDNF-TrkB通路的作用, 一定环境下, 二者平衡打破, 可能对细胞生长或增殖产生不同影响。 |
| 中文关键词:脑源性神经营养因子前体 p75神经营养因子受体 C6细胞 |
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| ProBDNF- p75NTR Pathway Inhibits C6 Cells Proliferation |
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| Abstract:Objective ProBDNF and mature BDNF play opposing roles through p75NTR receptor and TrkB receptor. The imbalance between proBDNF-p75 pathway and mature BDNF-TrkB pathway under some microenvironment may impact cell growth and proliferation differently. In the study the expression of proBDNF and its receptor p75NTR was investigated in C6 cells cultured in medium containing different concentration of FBS. The potential roles of proBDNF and p75NTR to C6 cells growth and proliferation were also focused under different culture medium. Methods The expression of proBDNF and its receptor-p75NTR was assayed in C6 cells cultured in medium containing different concentration of FBS by immunofluorescence double labelling and Western blot methods. C6 cells were treated with specific proBDNF antibody and p75NTR-ECD-Fc blocker. MTT and Brdu assays were performed to investigate the effects of proBDNF and p75NTR on C6 cells growth and proliferation. Results The coexpression of proBDNF and p75NTR was significantly upregulated in C6 cells cultured in FBS free medium. The C6 glioma cells growth and proliferation were increased after treatment with proBDNF antibody (10μg/ml) in vitro. The role of p75NTR may depend on which ligands it binds under different circumstances. Conclusion These findings in vitro suggest that proBDNF-p75 pathway may be a balancing signal to mature BDNF-TrkB pathway. The balance broken under certain circumstances may contribute to diverse role in cell growth and proliferation. |
| keywords:Precursor of brain derived neurotrophic factor p75 neurotrophin receptor C6 cell |
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