| 二苯乙烯苷抑制肺动脉平滑肌细胞增殖的机制研究 |
| 投稿时间：2014-09-24 修订日期：2014-10-13 点此下载全文 |
| 引用本文：官文俊,许臣洪.二苯乙烯苷抑制肺动脉平滑肌细胞增殖的机制研究[J].医学研究杂志,2015,44(6):122-126 |
| DOI：
10.11969/j.issn.1673-548X.2015.06.035 |
| 摘要点击次数: 1493 |
| 全文下载次数: 1356 |
|
|
| 中文摘要:目的 建立PDGF-BB诱导的原代大鼠肺动脉平滑肌细胞(pulmonary vascular smooth muscle cells,PASMCs)增殖的细胞模型,并探讨二苯乙烯苷(2,3,5,4-tetrahydroxyl diphenylethylene-2-o-glucoside,TSG)对PASMCs增殖的影响及其机制,为肺血管重构防治寻找新药物。方法 采用酶消化法分离培养大鼠原代PASMCs,通过20ng/ml PDGF-BB诱导PASMCs增殖建立细胞模型,采用1~100μmol/L TSG干预PDGF-BB诱导的PASMCs增殖,通过CCK-8检测PASMCs增殖及TSG的细胞毒作用,BRDU检测DNA的合成,流式细胞仪分析细胞周期,实时定量PCR(RT-PCR)检测CyclinD1、CyclinE、CDK2/4/6 mRNA的表达,Western blot法检测总的和磷酸化AKT/GSK3β的表达。结果 CCK-8检测结果表明TSG抑制PDGF-BB诱导的PASMCs增殖及DNA合成具有浓度依赖性,并且实验浓度的TSG对PASMCs无明显毒性不良反应;流式细胞仪分析结果表明TSG能够阻滞细胞周期于G0/G1~S期,RT-PCR结果表明TSG能够抑制CyclinD1、CyclinE、CDK2/4/6 mRNA的表达;Western blot法检测结果表明TSG能够抑制AKT/GSK3β活化。结论 TSG通过阻滞细胞周期于G0/G1~S抑制PDGF-BB诱导的PASMCs增殖。TSG抑制PASMCs增殖与抑制AKT/GSK3β信号通路的活化有关。 |
| 中文关键词:二苯乙烯苷 血小板源性生长因子 肺动脉平滑肌细胞 细胞周期 增殖 |
| |
| 2,3,5,4-Tetrahydroxyl Diphenylethylene-2-o-glucoside Blocked the Proliferation of Pulmonary Artery Smooth Muscle Cells Induced by Platelet-Derived Growth Factor-BB |
|
|
| Abstract:Objective To establish the platelet-derived growth factor-BB (PDGF-BB)-induced model of cell proliferation in rat pulmonary artery smooth muscle cells(PASMCs), and investigate the effects of 2,3,5,4-tetrahydroxyl diphenylethylene-2-o-glucoside(TSG)on the proliferation of PASMCs induced by PDGF-BB, as to search for new drugs for the treatment of pulmonary vascular remodeling. Methods Enzymatic digestion was used to separate the PASMCs and use PDGF-BB to induced the proliferation of PASMCs, and the effect of TSG on the proliferation of PASMCs was observed, After PDGF-BB treatment for 24 h, the proliferation was detectedby CCK-8, cell cycleby, flow cytometry at the mRNA expression of Cyclin D1, CyclinE and CDK2/4/6 by RFPCR. After PDGF-BB treatment for 15 min, the total and phosphorylated forms AKT, proto-synthase kinase 3β (GSK3β) was observed by Western Blot. Results CCK-8 test results showed that TSG blocked PDGF-BB-induced proliferation of PASMCs; flow cytometry analysis indicated that TSG arrested the cell cycle in G0/G1 to S phase. RT-PCR results demonstrated that TSG inhibited the mRNA expression of CyclinD1, CyclinE and CDK2/4/6. Western Blot indicated that TSG suppressed the activation of AKT/GSK3β signaling pathway. Conclusions TSG blocked the proliferation of PASMCs induced by PDGF-BB through arrestting the cell cycle in G0/G1 phase. Further study indicates that TSG suppress PDGF-BB-induced PASMCs proliferation is associated with an inhibition of AKT/GSK3β signal pathway. |
| keywords:2,3,5,4-Tetrahydroxyl diphenylethylene-2-o-glucoside Platelet-derived growth factor-BB Pulmonary artery smooth muscle cells Cell cycle Proliferation |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
