| PKCβ2途径抑制药物LY333531延缓高糖诱导心肌纤维化的实验研究 |
| 投稿时间：2015-03-31 修订日期：2015-04-13 点此下载全文 |
| 引用本文：苏文,苏兆林,陈晖,李虹伟,王萍.PKCβ2途径抑制药物LY333531延缓高糖诱导心肌纤维化的实验研究[J].医学研究杂志,2015,44(8):14-16 |
| DOI：
10.11969/j.issn.1673-548X.2015.08.006 |
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| 基金项目:国家自然科学基金资助项目(81100169);北京市自然科学基金资助项目(7122053) |
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| 中文摘要:目的 观察高糖对心脏成纤维细胞增殖及活化的影响及PKCβ2途径抑制剂LY333531的干预作用。 方法 将大鼠心脏成纤维细胞在正常糖或高糖环境下培养,检测两组细胞的增殖数量、胶原蛋白的合成及PKCβ2蛋白的表达,并检测使用LY333531干预后心脏成纤维细胞增殖及活化指标的变化。 结果 高糖可使PKCβ2蛋白表达增加,并能增加心脏成纤维细胞的增殖及Ⅰ型、Ⅲ型胶原蛋白的合成,LY333531阻断PKCβ2途径后可减少成纤维细胞增殖的数量及减少胶原蛋白的合成。 结论 LY333531作为干预药物抑制PKCβ2途径可以在一定程度上延缓高糖诱导的心肌纤维化过程。 |
| 中文关键词:PKCβ2途径 LY333531 高糖 心肌纤维化 |
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| PKCβ2 Pathway Inhibitor LY333531 Ameliorates High Glucose-induced Myocardial Fibrosis |
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| Abstract:Objective To observe the proliferation and activation of cardiac fibroblasts (CFs) in high glucose condition and explore the effect of PKCβ2 pathway inhibitor LY333531 on this process. Methods We cultured the rat CFs in normal glucose or high glucose media for a period of time, and observed the proliferation of CFs, the synthesis of collagen and the expression of PKCβ2. We incubated CFs with high glucose in combination of LY333531, and assessed the proliferation and activation of CFs. Results High glucose increased the expression of PKCβ2, and stimulated proliferation of CFs and synthesis of typeⅠand type Ⅲ collagen. LY333531 inhibited high glucose-mediated CFs' proliferation and collagen synthesis. Conclusion LY333531 can ameliorate high glucose-induced myocardial fibrosis by inhibiting PKCβ2 pathway. |
| keywords:PKCβ2 pathway LY333531 High glucose Cardiac fibrosis |
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