| HBeAg对小鼠骨髓源性树突状细胞表型及功能的影响 |
| 投稿时间：2014-11-21 修订日期：2014-12-29 点此下载全文 |
| 引用本文：蓝松松,吴乐灿,吴金明,王秀燕,林贤凡,吴文治,黄智铭,吴建胜.HBeAg对小鼠骨髓源性树突状细胞表型及功能的影响[J].医学研究杂志,2015,44(8):48-52 |
| DOI：
10.11969/j.issn.1673-548X.2015.08.015 |
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| 基金项目:浙江省自然科学基金资助项目(LY12H03003;Y2110768);温州市科技计划项目(2014Y0136) |
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| 中文摘要:目的 研究HBeAg对小鼠骨髓源性树突状细胞(dendritic cell,DC)表型及功能的影响。 方法 以rmGM-CSF和rmIL-4定向体外诱导C57BL/6小鼠骨髓细胞分化成未成熟DC,随机分为空白对照组、HBeAg刺激组、脂多糖(LPS)刺激组和HBeAg+LPS刺激组。以流式细胞术检测DC表型变化,混合淋巴反应(MLR)检测DC促T淋巴细胞增殖能力,酶联免疫法(ELISA)检测细胞上清液中IL-12的分泌水平,CCK-8法检测HBeAg对骨髓源性树突状细胞活力影响。 结果 HBeAg刺激后,CD11c阳性细胞百分数下降。 HBeAg可抑制DC表面MHC-Ⅱ、CD86的表达和DC促淋巴细胞增殖的能力,且HBeAg可抑制LPS诱导的树突状细胞IL-12的分泌,细胞活力检测显示HBeAg对细胞没有明显毒性作用。 结论 HBeAg对树突状细胞的成熟有一定的负性调节作用,这可能是HBV的持续感染的机制之一。 |
| 中文关键词:乙型肝炎e抗原 树突状细胞 脂多糖 表面分子 白细胞介素12 |
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| Effects of HBeAg on the Phenotype and Function of Murine Bone Marrow-derived Dendritic Cells |
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| Abstract:Objective To investigate the effect of HBeAg on dendritic cell phenotype and function of murine bone marrow-derived DCs. Methods The murine bone marrow cells were cultured and induced in vitro into immature DCs. These cells were divided into the control group, HBeAg-stimulated group,LPS-stimulated group, LPS plus HBeAg-treated group. Then,immunophenotyping, T-cell activation and cytokine production were analyzed. In addition,the HBeAg cytotoxic effect was evaluated by detection of the cell viability. Results In the presence of HBeAg,the ratio of CD11c positive DCs was decreased. LPS induced a high expression of activation markers (CD86,MHC-Ⅱ) and a enhanced capacity to stimulate allogeneic T cell proliferation. HBeAg could suppress the the expression of surface molecules. Also, HBeAg decreased the ability of DCs on stimulating leukomonocyte and secreting IL-12.And HBeAg did not reveal any cytotoxicity on DCs. Conclusion Our findings indicated that HBeAg may have a negative effect on the maturation of DCs. |
| keywords:Hepatitis B e antigen Dendritic cell LPS Surface molecule Interleukin-12 |
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