| 宫颈癌HeLa细胞抗顺铂作用的Notch1机制研究 |
| 投稿时间：2015-01-29 修订日期：2015-03-06 点此下载全文 |
| 引用本文：杨晶,张军红,马慧娟,王志华,王琼,王川.宫颈癌HeLa细胞抗顺铂作用的Notch1机制研究[J].医学研究杂志,2015,44(10):34-37 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.010 |
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| 基金项目:国家自然科学基金资助项目(31171097);国家自然科学基金资助项目(青年项目)(81100229);河北省自然科学基金资助项目(C2014206419);河北省高等学校科学技术研究项目(ZD2015007);邢台市科技支撑计划项目(2013ZC062);河北省卫生厅医学研究重要课题(20090319);河北省科技厅科学技术研究与发展计划项目(11246125D) |
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| 中文摘要:目的 利用宫颈癌HeLa细胞系探讨宫颈癌的抗化疗机制。 方法 利用球形成试验(sphere forming assay)和Western blot法检测顺铂对癌症干细胞形成的影响以及Notch1在HeLa细胞系的表达。基因沉默试验分析Notch1基因是否在宫颈癌HeLa癌症干细胞中发挥关键作用。 结果 顺铂能促进球结构(意味着癌症干细胞形成)的形成。5μmol/L顺铂较1μmol/L顺铂能促进更多球结构形成(P<0.05)。10μmol/L顺铂会抑制球结构形成。5μmol/L顺铂还能提高HeLa 中Notch1的表达(P<0.05),而沉默HeLa细胞的Notch1后,HeLa细胞中形成的球数量降低1.7倍(P<0.05)。 结论 宫颈癌HeLa细胞系具有抗顺铂治疗的特性,尤其是5μmol/L的顺铂,该抗化疗作用与宫颈癌HeLa细胞中的Notch1表达升高,最终促进癌症干细胞自我更新有关。 |
| 中文关键词:癌症干细胞 Notch1 宫颈癌 HeLa细胞系 |
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| Notch1 Mediates the Anti-cisplatin Character of Cervical Cancer LeLa Cell Line |
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| Abstract:Objective To explore the anti-chemotherapy mechanism of cervical cancer using the cervical cancer HeLa cell line. Methods Sphere forming assay and Western blotting were used to measure the potential effects of Cisplatin on cancer stem cell formation and Notch1 expression in HeLa cells. Gene silencing assay was performed to analyze whether Notch1 played a key role in the proliferation of HeLa cancer stem cells. Results Cispaltin basically promoted the formation of ball structures (an indicator of cancer stem cells) except at larger concentration (10μmol/L).Cispaltin at 5μmol/L exerted greater promoting effect on the ball structures compared with that at 1μmol/L (P<0.05). Cispaltin at 10μmol/L inhibited the formation of ball structures. Cispaltin at 5μmol/L also promoted Notch1 expression in HeLa cells (P<0.05), while silencing Notch1 decreased ball structure formation by 1.7 folds (P<0.05). Conclusion Cervical cancer HeLa cell line behaves an character of anti-Cispaltin therapy especially at 5μmol/L, and this tendency is likely associated the promoting effect of Cisplatin on the expression of the self-renewal factor Notch1 and proliferation of cancer stem cells. |
| keywords:Cancer stem cell Notch1 Cervical cancer HeLa cell line |
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