| 中期因子对骨折愈合过程微血管生成影响的实验研究 |
| 投稿时间：2015-01-08 修订日期：2015-02-23 点此下载全文 |
| 引用本文：李雄峰,李建有,周国顺,管国华.中期因子对骨折愈合过程微血管生成影响的实验研究[J].医学研究杂志,2015,44(10):46-50 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.013 |
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| 基金项目:浙江省医药卫生科技项目(2009B153);湖州市科技计划项目(2009YS04) |
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| 中文摘要:目的 探讨中期因子对骨折端微血管生成对骨折愈合的影响。 方法 采用兔尺骨骨折模型,给以中期因子持续刺激骨折端7天为实验组,给予生理盐水刺激的为对照组,然后在21、42、63、84天,分别获取标本。采用组织学Lane-sandhu评分分析骨组织的生成;免疫组化分析骨折端VEGF和CD34表达来探讨骨折端的微血管生长情况,采用图像分析和MVD评分进行统计分析。 结果 组织学Lane-sandhu评分在42和63天实验组明显高于对照组,VEGF表达在42和63天实验组明显高于对照组;而CD34在42、63、84天周实验组明显高于对照组。 结论 中期因子通过刺激骨折端微血管增生增加是骨折端骨痂生长加快的原因,有利于骨折愈合。 |
| 中文关键词:中期因子 骨折 微血管 血管内皮生长因子 |
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| Expression of Vascular Endothelial Growth Factor and Effects in Bone Healing Process byMidkine on Rabbit Fracture Model |
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| Abstract:Objective To explore the angiogenesis in bone healing process stimulated by midkine on rabbit fracture model. Methods Rabbit Ulna fracture model, putted MK gelatin sponge to the bone fracture defects and inject MK everyday continuing one week on experimental group, control group just use physiological saline gelatin sponge inject physiological saline at same time. Harvested the examples at 21,42,63,84 days respectively. Bone formation was evaluated according to the Lane-Sandhu criteria, analyzed the expression of VEGF and CD34 by immunohistochemisty. Results According to the Lane-Sandhu criteria,we found that at 42 days and 63 days the bone healing of experimental group was better than control group, as same as the expression of VEGF. The expression of CD34 in experimental group was better than control group at 21,42,63 days respectively. Conclusion We considered that MK promoting the bone formation in rabbit fracture model by induce neovascularization. It could be used to enhanced the healing of fracture. |
| keywords:Midkine Fracture Angiogenesis VEGF |
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