| 人参皂甙Rg3对胰腺癌裸鼠皮下移植瘤血管生成拟态的研究 |
| 投稿时间：2014-12-13 修订日期：2015-01-06 点此下载全文 |
| 引用本文：江丹丹,郭敬强,陈亮.人参皂甙Rg3对胰腺癌裸鼠皮下移植瘤血管生成拟态的研究[J].医学研究杂志,2015,44(10):124-128 |
| DOI：
10.11969/j.issn.1673-548X.2015.10.035 |
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| 中文摘要:目的 本实验通过建立胰腺癌SW-1990细胞株皮下移植瘤模型,探讨人参皂甙Rg3对胰腺癌皮下移植瘤血管生成拟态的影响。 方法 首先建立人胰腺癌细胞裸鼠模型,后给予不同药物浓度的人参皂甙Rg3(0、5、10、20mg/kg)处理裸鼠,观察人参皂甙Rg3对裸鼠移植瘤生长的影响,免疫组化-PAS双染观察人参皂甙Rg3对裸鼠胰腺癌皮下移植瘤血管生成拟态和血管内皮因子CD31的影响,并用荧光定量PCR和Western blot法分别检测MMP-2、MMP-9在mRNA和蛋白水平的表达。 结果 人参皂甙Rg3能够抑制胰腺癌裸鼠皮下移植瘤的生长,其中20mg/kg人参皂甙Rg3组最明显;Western blot法和荧光定量PCR示用药组与空白组比较,MMP-2、MMP-9在蛋白和mRNA水平的表达下降,且差异有统计学意义(P<0.05);免疫组化-PAS双染后发现血管拟生(+)和CD31(+)数量均较空白组减少。 结论 通过体内实验笔者证实人参皂甙Rg3能够通过降低MMP-2、MMP-9的表达来抑制胰腺癌血管生成拟态的形成。 |
| 中文关键词:胰腺癌 胰腺癌裸鼠皮下移植瘤 血管生成拟态 基质金属蛋白酶 |
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| Effects of Ginsenosides Rg3 on Vasculogenic Mimicry of Pancreatic Cancer Xenograft |
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| Abstract:Objective To investigate the effects of ginsenosides Rg3 on vasculogenic mimicry of pancreatic cancer xenograft through the establishment of pancreatic cancer cell line SW-1990 subcutaneous xenograft model. Methods After pancreatic cancer xenograft in nude mice model beening established, All the mice were randomly divided into 4 groups and treated intraperitoneally (IP) with saline and various concentrations (5,10,20 mg/kg) of ginsenosides Rg3. To observe the effect of ginsenoside Rg3 on tumor growth. Immunohistochemical-PAS staining was used to detect the effects of ginsenosides Rg3 on vasculogenic mimicry of pancreatic cancer xenograft. and mRNA and protein expression of MMP2、MMP9 were respectively evaluated by FQ-PCR and Western blot. Results The ginsenosides Rg3 can inhibit the growth of the tumor xenografts in nude mice. The inhibitory effect is the most obvious the 20 mg/kg of ginsenosides Rg3 group. The expression of MMP-2, MMP-9 were down-regulated compare with the control group, and the difference was significant; the Immunohistochemical-PAS staining showed the number of vasculogenic mimicry (+) and CD31 (+) were less than that in the control group. Conlusion Our results demonstrate that pancreatic vascular mimicry formation can be suppressed by Ginsenoside Rg3 though reducing the expression of MMP-2, MMP-9 in our vivo experiments. |
| keywords:Pancreatic cancer Xenograft model of pancreatic cancer Vasculogenic mimicry Matrix metalloproteinase |
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