| 细菌显像剂18F-FDS的合成及动物显像应用研究 |
| 投稿时间：2015-08-03 修订日期：2015-09-06 点此下载全文 |
| 引用本文：邢海群,朱文佳,蔡炯,张迎强,要少波,霍力,李方.细菌显像剂18F-FDS的合成及动物显像应用研究[J].医学研究杂志,2016,45(1):51-54 |
| DOI：
10.11969/j.issn.1673-548X.2016.01.013 |
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| 基金项目:国家自然科学基金资助项目(面上项目)(81571712) |
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| 中文摘要:目的 探讨细菌显像剂2-脱氧-2-氟山梨醇(2-deoxy-2-[18F]-fluorosorbitol, 18F-FDS)的合成过程、正常小鼠显像并计算内照射剂量,比较小鼠模型中大肠杆菌感染病灶及肿瘤病灶对FDS的摄取情况。方法 18F-FDS由氟多功能模块经"一锅法"由PET常用显像剂2-氟-2脱氧葡萄糖(18F-fluorodeoxyglucose, 18F-FDG)经NaBH4还原制备;实验组感染小鼠及荷瘤鼠尾静脉注射18F-FDS后60min进行microPET显像,通过Inveron Research软件测量病灶靶/本底比值;对照组注射18F-FDG,同样方法测量病灶靶/本底比值进行对照分析。结果 18F-FDS放化收率为80%±5%(n=8,未进行时间衰减校正),合成时间25min,放化纯度>99%。正常小鼠尾静脉注射18F-FDS后microPET显像提示示踪剂从泌尿系统排泄;背景组织肺及脑内的非特异性放射性摄取较低;大肠杆菌感染小鼠microPET显像,感染灶有较高的感染灶/肌肉比值,荷瘤鼠显像示18F-FDS肿瘤组织靶/本底比值明显低于18F-FDG。结论 18F-FDS放化合成方法操作简单,收率高,细菌感染灶有特异性摄取,在分辨感染和肿瘤上具有应用前景。 |
| 中文关键词:感染 18F-FDS 大肠杆菌 肿瘤 放化标记 |
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| Radiosynthesis of Infection Tracer 18F-FDS and Animal Imaging |
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| Abstract:Objective To synthesis 18F-FDS from 18F-FDG, calculate internal radiation dose in normal mice imaging, look for the difference of FDS accumulated in E.coli infection mouse model and in tumor lesions. Methods 18F-FDS was synthesized on multifunctional synthesis module from 18F-FDG with sodium borohydride reduction by 'One-pot reaction'. In test group, 18F-FDS was injected into infection mice model and tumor mice model via tail vein for microPET imaging after 60min. T/B values of 18F-FDS were measured and analyzed by Inveron Research software. 18F-FDG was injected into model mice in control group and T/B values were measured as describe above for comparison. Results It took 25 min to complete 18F-FDS synthesis. The radiochemical yield and radiochemistry purity of 18F-FDS was 80%±5%, and over 99% respectively. MicroPET imaging data with normal mouse suggested that 18F-FDS was cleared from kidneys. The nonspecific uptake of 18F-FDS in background tissue such as lung, brain was very low. MicroPET imaging data showed that E. coli infected loci in mouse model had high T/B ratio, while tumor lesions had much lower T/B ratio. Conclusion 18F-FDS is easy to be prepared with high yield and can be used to trace bacteria infected loci specifically. It is a promising radiotracer for differentiated diagnosis of bacteria infection from tumor. |
| keywords:Infection 18F-FDS E.coli Tumor Radiolabeling |
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