| PDE5基因沉默对肾透明细胞癌细胞增殖和凋亡的影响 |
| 投稿时间：2015-08-30 修订日期：2015-09-18 点此下载全文 |
| 引用本文：傅晶,陈尧康,虞利民,何小祥,黄正,叶华建.PDE5基因沉默对肾透明细胞癌细胞增殖和凋亡的影响[J].医学研究杂志,2016,45(3):112-115 |
| DOI：
10.11969/j.issn.1673-548X.2016.03.029 |
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| 基金项目:绍兴市科技计划项目(2010D10014) |
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| 中文摘要:目的 探讨PDE5基因对肾透明细胞癌细胞株786-O细胞增殖和凋亡的影响.方法 设计、合成PDE5 siRNA序列,按照HiPerFect转染试剂盒操作手册转染786-O细胞.采用实时定量聚合酶链反应(real-time quantitative PCR)和Western blot法检测转染后PDE5 基因的表达,通过WST-1法检测细胞增殖情况,caspase-3活性检测试剂盒检测caspase-3活性.结果 与空白对照组和阴性对照组相比较,PDE5 siRNA转染组的mRNA及蛋白表达水平显著降低,786-O细胞增殖能力明显受到抑制,caspase-3活性明显增加,差异均具有统计学意义(P<0.05).结论 siRNA干扰PDE5基因表达可抑制肾透明细胞癌细胞株786-O细胞的增殖并促进凋亡. |
| 中文关键词:肾透明细胞癌 PDE5基因 细胞增殖 细胞凋亡 |
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| Effects of PDE5 Gene Silence on the Proliferation and Apoptosis of Clear Cell Renal Cell Carcinoma Cells. |
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| Abstract:Objective To explore the effect of PDE5 gene on the proliferation and apoptosis of clear cell renal cell carcinoma cell line 786-O. Methods The siRNA of PDE5 was constructed and transfected into 786-O cells with HiPerFect transfection reagent. The PDE5 expression levels after transfection were detected by using real-time quantitative PCR and Western blot. The cell proliferation was assessed by WST-1 assay. The caspase-3 activity was detected by kit. Results Compared with the control group and blank group, the mRNA and protein levels were significantly reduced and the proliferation of 786-O cells was significantly inhibited in the PDE5 siRNA group (P<0.05), while caspase-3 activity was significantly increased (P<0.05). Conclusion siRNA interference of PDE5 gene may inhibit cell proliferation and promote cell apoptosis in clear cell renal cell carcinoma cell line 786-O. |
| keywords:Clear cell renal cell carcinoma PDE5 gene Cell proliferation Cell apoptosis |
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