| 结核分枝杆菌恒温荧光检测技术临床应用评价研究 |
| 投稿时间：2015-12-29 修订日期：2016-01-04 点此下载全文 |
| 引用本文：欧喜超,夏辉,逄宇,李强,赵冰,董海燕,张治英,赵雁林.结核分枝杆菌恒温荧光检测技术临床应用评价研究[J].医学研究杂志,2016,45(6):63-66 |
| DOI：
10.11969/j.issn.1673-548X.2016.06.016 |
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| 基金项目:中国国家卫生与计划生育委员会与比尔及梅琳达·盖茨基金会结核病防治项目（2009-04-01） |
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| 中文摘要:目的 评估恒温荧光（RealAmp）检测技术在结核病临床诊断中的应用价值。方法 2013年5～8月，在上海市肺科医院、山东省胸科医院和广州市胸科医院，门诊医生连续纳入初诊肺结核可疑症状者，每例患者留取1份合格痰标本。实验室人员对痰标本同时进行涂片染色荧光显微镜镜检、液体培养和RealAmp检测，培养阳性菌株进行16S-23S间隔区测序菌群鉴定。以临床诊断结果为金标准，比较涂片镜检、液体培养和RealAmp检测结核分枝杆菌的敏感度和特异性。结果 与临床诊断结果比较，涂片镜检、液体培养和RealAmp检测结核分枝杆菌的敏感度分别为17.16%、39.45%和39.72%，RealAmp检测特异性为91.61%。RealAmp检测敏感度高于涂片镜检（χ2=134.42，P<0.05），与液体培养试验相当。结论 RealAmp检测操作简单，敏感度较高，能够提高涂阴肺结核患者的检出。 |
| 中文关键词:结核分枝杆菌 恒温荧光 扩增诊断 |
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| Clinical Evaluation Study of Isothermal Fluorescence Detection Method for Mycobacterium Tuberculosis Detection |
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| Abstract:Objective To evaluatethe clinical application value of isothermal fluorescence detection methodin the diagnosis of TB suspects. Methods From May 2013 to August 2013, clinician continuously recruited patients with suspected tuberculosis from three chest hospitals in shanghai, shandong and guangzhou. Each patient provided one qualified sputum specimen. Laboratory staff performed microscopic examination by fluorescence microscope, liquid culture and RealAmpfor thisspecimen.All positive cultures underwent 16S-23S rDNA ITS sequencing for specie confirmation.Using the clinical diagnosis as reference standard, we evaluate the performance of RealAmp test for mycobacterium tuberculosis detection. Results Compared with clinical diagnosis, the sensitivity of smear microscopy, solid culture and RealAmp were 17.16%, 39.45% and 39.72%, respectively, the specificity of RealAmp was 91.61%. The sensitivity of RealAmp was higher than smear (chi-square=134.42, P<0.05) and approximate with the liquid culture. Conclusion The operation of RealAmp test is simple and the sensitivity is higher. It can increase the detection rate of smear negative TB patients. |
| keywords:Mycobacterium tuberculosis RealAmp Diagnosis |
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