| Abstract:Objective To investigate the protective effects of Ginkgolide B(GB) on H9C2 cardiomyocytes impared by H2O2 and its mechanism. Methods H9C2 cardiomyocytes in logarithmic phase was randomly divided into normal control group, H2O2(200μg/ml) group, GB(100μg/ml)+H2O2(200μg/ml) group, GB(200 μg/ml)+H2O2(200μg/ml) group (n=10). Sixteen hours after the drugs were given, the morphology changes was observed by optical microscope, the survival rate was detected by MTT. the activity of AST, CPK, LDH in culture medium were detected, the activity of SOD, GSH-Px, CAT and the content of MDA in cardiomyocytes were determinted, the content level of TNF-α, IL-1, IL-6, CRP were detected by ELISA, the cardiomyocytes apoptosis was observed by flow cytometry and the apoptosis rate was calculated, the expression of bcl-2 mRNA, Bax mRNA were detected by RT-PCR, and the ratio of bcl-2/Bax was calculated, the expression of NF-κB proten was determined and semi-quantitative analysized. Results Compared with the H2O2group, the activity of AST, CPK and the content of TNF-α, IL-6, CRP, MDA in culture medium of GB(100, 200μg/ml)+H2O2groups were significantly decreased(P<0.05, P<0.01). The activity of SOD, CAT in cardiomyocytes were significantly increased(P<0.05, P<0.01). The apoptosis rate was significantly decreased(P<0.01). The expression of bcl-2 mRNA was significantly up-regulated, the expression of Bax mRNA was significantly down-regulated, and the ratio of bcl-2/Bax were significantly increased(P<0.01). The morphology of cardiomyocytes in GB(200μg/ml)+H2O2groups was improved and the survival rate was significantly increased(P<0.01). The activity of LDH, the content of IL-1 and the expression of NF-κB proten were significantly decreased(P<0.05,P<0.01). The activity of GSH-Px was significantly increased(P<0.05). Conclusion GB had protective effects on H9C2 cardiomyocytes impared by H2O2, which perhaps related to its effects of improving the activity of antioxidase, depressing oxidative stress, up-regulating the expression of bcl-2, down-regulating the expression of Bax, raising the ratio of bcl-2/Bax, down-regulating the expression of NF-κB, lowering the content of inflammatory cytokines. |
