| 低氧抑制人脐静脉血管内皮细胞miR-26a的表达 |
| 投稿时间：2015-11-02 修订日期：2015-11-20 点此下载全文 |
| 引用本文：俞楠泽,龙飞,贾毅弘,王晓军,龙笑.低氧抑制人脐静脉血管内皮细胞miR-26a的表达[J].医学研究杂志,2016,45(8):55-58 |
| DOI：
10.11969/j.issn.1673-548X.2016.08.015 |
| 摘要点击次数: 1568 |
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| 基金项目:国家自然科学基金青年科学基金资助项目（81401600）；中国医学科学院协和青年科研基金资助项目（A101100） |
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| 中文摘要:目的 观察低氧对人脐静脉血管内皮细胞（HUVEC）miR-26a及PTEN/AKT/VEGF通路相关因子表达的影响。方法 分常氧组和低氧组培养48h。荧光定量RT-PCR和Western blot法检测miR-26a及PTEN/AKT/VEGF通路相关因子的表达。结果 RT-PCR结果显示低氧组HUVEC中miR-26a和PTEN的相对表达量为0.42±0.02和0.62±0.03，显著低于常氧组的1.00±0.34和1.00±0.35（P<0.05）。Western blot法检测结果显示，与常氧组相比，乏氧组HIF-1、VEGF和AKT蛋白表达明显升高，PTEN 蛋白明显降低（P<0.05）。结论 低氧抑制HUVEC miR-26a的表达并继发下游PTEN/AKT/VEGF因子的序贯反应，可作为创面改善缺血状态治疗的新靶点。 |
| 中文关键词:低氧 人脐静脉血管内皮细胞 miR-26a PTEN |
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| Hypoxia Inhibits MiR-26a Expression in Human Umbilical Vein Endothelial Cells (HUVEC) |
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| Abstract:Objective To observe the miR-26a expression and PTEN/AKT/VEGF pathway factor expression of human umbilical vein endothelial cells (HUVEC) under hypoxia condition. Methods HUVEC were divided into normoxia group and hypoxia group. RT-PCR was used to detect differences of miR-26a and PTEN expression between the groups, and the expression of PTEN/AKT/VEGF pathway factors at the protein level was detected by Western blot. Results After 48 hours, the cells in the hypoxic group expressed much lower miR-26a and PTEN than the normoxia group at the RNA level (P<0.05). Western blot analysis showed that the hypoxic group expressed increased HIF-1 and VEGF protein level and decreased AKT and PTEN protein level. Conclusion Low expression of miR-26a can trigger PTEN/AKT/VEGF pathway factor chain reaction in HUVEC under hypoxia conditions in vitro, which indicates miR-26 may be a potential treatment target for chronic ischemic wounds. |
| keywords:Under hypoxia Human umbilical vein endothelial cells (HUVEC) MiR-26a PTEN |
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