| miR-577在胃癌中的表达及对SGC-7901细胞侵袭的影响 |
| 投稿时间：2016-03-23 修订日期：2016-03-30 点此下载全文 |
| 引用本文：何银平,叶景旺.miR-577在胃癌中的表达及对SGC-7901细胞侵袭的影响[J].医学研究杂志,2016,45(10):95-98 |
| DOI：
10.11969/j.issn.1673-548X.2016.10.025 |
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| 中文摘要:目的 研究微小RNA 577（microRNA-577，miR-577）在人胃癌（gastric cancer，GC）组织内的表达情况及对肿瘤细胞侵袭能力的影响。方法 选择2013年1月~2015年3月于第三军医大学大坪医院普通外科收治并行手术切除的70例胃癌与对应癌旁组织。通过qRT-PCR方法检测miR-577在组织中的相对表达量，分析miR-577的临床病理意义；采用人工合成的miR-577模拟物（miR-577 mimics）转染人胃癌SGC-7901细胞，并检测SGC-7901细胞过表达miR-577后侵袭能力及miR-577下游潜在靶点β-catenin mRNA及蛋白的表达变化。结果 MiR-577在胃癌组织中表达显著下调（P=0.000）并与与淋巴结转移（P=0.003）、高TNM分期（P=0.006）等不良临床特征呈正相关；过表达miR-577能够显著抑制SGC-7901细胞侵袭能力（P=0.000）及细胞内β-catenin的表达水平。结论 miR-577在胃癌中低表达，并可能通过下调β-catenin的表达来抑制胃癌细胞的侵袭。 |
| 中文关键词:MicroRNA-577 胃癌 β-catenin 侵袭 |
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| Expression of miR-577 and Its Role for Cell Invasion in Human Gastric Carcinoma |
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| Abstract:Objective To identify the level of microRNA-577 and its effect for cell invasion in human gastric carcinoma(GC). Methods The level of miR-577 in 70 paired GC and matched non-tumor tissues collecting between January 2011 and March 2015 was detected by qRT-PCR. We then transfected the miR-577 mimics into SGC-7901 cells. Cell invasion ability was measured by transwell assay. qRT-PCR and western-blot were used to detect the expression of β-catenin, a potential target of miR-577, in transfected SGC-7901 cells. Results miR-577 was low expression in gastric cancer tissues and correlated with lymphatic metastasis(P=0.003) and advanced TNM stage(P=0.006). Transfection of miR-577 mimics could suppress cell invasion(P=0.000) and inhibit the expression of β-catenin (P=0.000). Conclusion miR-577 was low expression in GC tissues, and miR-577 could suppress GC invasion through down-regulating β-catenin. |
| keywords:mircoRNA-577 Gastric cancer β-catenin Invasion |
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