| 高脂血症小鼠铁调素表达与肝功能损伤的关系 |
| 投稿时间：2016-02-17 修订日期：2016-04-08 点此下载全文 |
| 引用本文：王琳,马晓娟,孙明月,苗洋,刘欣,张淼,刘金明,张颖,殷惠军.高脂血症小鼠铁调素表达与肝功能损伤的关系[J].医学研究杂志,2016,45(11):54-57 |
| DOI：
10.11969/j.issn.1673-548X.2016.11.014 |
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| 作者 | 单位 | E-mail | | 王琳 | 730000 兰州, 甘肃中医药大学 | | | 马晓娟 | 100091 北京, 中国中医科学院西苑医院 | | | 孙明月 | 100091 北京, 中国中医科学院西苑医院 | | | 苗洋 | 100091 北京, 中国中医科学院西苑医院 | | | 刘欣 | 100091 北京, 中国中医科学院西苑医院 | | | 张淼 | 100091 北京, 中国中医科学院西苑医院 | | | 刘金明 | 730000 兰州, 甘肃中医药大学 | | | 张颖 | 100091 北京, 中国中医科学院西苑医院 | doctoronline@126.com | | 殷惠军 | 730000 兰州, 甘肃中医药大学
100091 北京, 中国中医科学院西苑医院 | huijunyin@yeah.net |
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| 基金项目:国家自然科学基金资助项目（81173584，81202837）；甘肃高校飞天学者计划（甘教厅2013-88号） |
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| 中文摘要:目的 观察高脂血症小鼠血清铁调素表达变化，探讨肝功能损伤与高脂血症小鼠铁调素的相关性。方法 60只4周龄雄性C57BL/6J小鼠随机分为6组，每组10只，分别为0、2、4、6、8周组以及8周正常对照组，造模组40只给予脂肪乳灌胃，分别在2、4、6、8周处死，复制高脂血症伴有肝脏损伤模型。对照组给予等体积生理盐水，造模成功后，取血分离肝脏。全自动生化分析仪检测血脂（TC、TG、LDL-C、HDL-C）、肝功能（ALT、AST）、血清铁（FER、TRF）。血清酶联免疫吸附法测：血清铁调素（hepcidin）水平。结果 与空白组比较，模型组TC、TG水平明显升高（P<0.05）造模成功，2周组小鼠hepcidin表达水平明显下降（P<0.05）。与空白组比较，小鼠铁调素（hepcidin）表达水平随高脂喂养时间延长，铁调素表达有升高趋势，以8周组下降差异有统计学意义（P<0.05）。血清铁调素与肝功能损伤程度间呈负性相关（r=0.878，P<0.01）。结论 高脂血症小鼠血清铁调素表达随血脂水平升高而增多。伴随肝功能受损程度加重，铁调素表达下降，表明铁调素水平能反映肝脏损害程度。 |
| 中文关键词:铁调素 高脂血症 肝功能损伤 小鼠 |
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| Correlation between Hepcidin Expression and Liver Dysfunction in Hyperlipidemic Mice |
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| Abstract:Objective To investigate the level of hepcidin on the hyperlipidemia model induced by lipid emulsion, researching the relationship between liver function with changed hepcidin. Methods Aged 4 weeks sixty male C57BL/6J mice were randomly divided into control and model groups,with in 10per group. Expect that 10 mice as control group were sacrificed and 10 mice were intragastrically administered normal saline, others were intragastrically given Fat-emulsion respectively for 2,4,6,8 weeks to generate the hyperlipidemia with liver damage model. After 8 weeks, all established mice were anesthetized. Blood was collected in sterile tubes. Liver were removed and sacrificed by cervical dislocation. Serum lipids parameters were measured with an automated analyzer. hepcidin levels were determined using an enzyme linked immunosorbent assay. Results Compared with the control group, all treatment group TC/TG/LDL-C/hepcidin had significantly decrease(P<0.05),especially for 8-week group(P<0.01).From the pathology,we can observe the change with necrosis and steatosis(P<0.05). Conclusion The hepcidin protein may be inhibited by dysfunction of liver, comparative with the expression of hepcidin affect by liver function. All the results may give a direct evidence to differentiation of syndrome and provide new target to therapy and clinical laboratory and to access the prognosis of hyperlipidemia. |
| keywords:Hepcidin Hyperlipidemia Liver dysfunction Mice |
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