| lincRNA-p21抑制肾癌细胞增殖作用 |
| 投稿时间：2016-06-20 修订日期：2016-07-30 点此下载全文 |
| 引用本文：潘珠玛,黄文瀚,李达学.lincRNA-p21抑制肾癌细胞增殖作用[J].医学研究杂志,2017,46(3):149-152 |
| DOI：
10.11969/j.issn.1673-548X.2017.03.037 |
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| 中文摘要:目的 探讨lincRNA-p21对肾癌细胞增殖作用的影响。方法 收集17例重庆市第七人民医院内分泌肾病科肾癌患者的组织样本，利用RT-PCR检测肾癌及其癌旁组织中lincRNA-p21的表达水平及人肾癌细胞系786-O、人胚肾细胞系HEK-293细胞中lincRNA-p21的表达水平，用siRNA降低lincRNA-p21的表达后观察其对细胞增殖、凋亡情况的影响以及对Bax、Noxa、Puma等p53信号通路中癌症相关基因表达水平的影响。结果 在肾癌患者的癌灶组织中，lincRNA-p21的表达水平明显高于癌旁组织（P<0.05），786-O较HEK-293细胞lincRNA-p21 mRNA表达水平显著升高（P<0.05）；用siRNA降低lincRNA-p21的表达后发现786-O细胞增殖增强，凋亡减弱，并且Bax、Noxa、Puma等癌症相关基因表达水平也被抑制（P<0.05）。结论 lincRNA-p21可能通过参与p53信号通路的转导，对肾癌细胞的增殖起到抑制作用，提示lincRNA-p21可作为分子靶点用于肾癌的靶向治疗。 |
| 中文关键词:lincRNA-p21 肾癌增殖 p53 |
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| Efffect of LincRNA-p21 on Proliferation of Renal Cell Carcinoma |
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| Abstract:Objective To study the effect of lincRNA-p21 on the proliferation of renal cell carcinoma. Methods The expression levels of lincRNA-p21in renal cell carcinoma tissues and their corresponding para-cancerous tissues from 17 patients in department of nephrology,Hua Xi hospital, was detected by RT-PCR. In addition, the expression of lincRNA-p21 was analyzed in human clear cell RCC cell line 786-O and human embryonic kidney cell line HEK-293. The cell proliferation, apoptosis and the expression of Bax、Noxa、Puma that participated in p53 signaling pathway were detected after lincRNA-p21 was downregulated with siRNA. Results The expression levels of lincRNA-p21was higher in renal cell carcinoma tissues and 786-O cells than para-cancerous tissues and HEK-293 cells respectively(P<0.05). The ability of proliferation was significantly increased after lincRNA-p21 siRNA treatment in 786-O cells, whereas the cell apoptosis was decreased(P<0.05). The mRNA expressions of Bax,Noxa,Puma were also dramatically decreased after lincRNA-p21 was downregulated(P<0.05). Conclusion LincRNA-p21 inhibits the cell proliferation of 786-O cells through participation in p53 signal transduction pathways. These data suggested that lincRNA-p21 maybe could be used as molecular targets for human RCC treatment. |
| keywords:LincRNA-p21 RCC Proliferation P53 |
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