| A型肉毒毒素对增生性瘢痕成纤维细胞增殖、凋亡及对ERK/MAPK信号通路的影响 |
| 投稿时间：2017-01-04 修订日期：2017-01-17 点此下载全文 |
| 引用本文：沈利,余扬,马少林.A型肉毒毒素对增生性瘢痕成纤维细胞增殖、凋亡及对ERK/MAPK信号通路的影响[J].医学研究杂志,2017,46(7):26-29,74 |
| DOI：
10.11969/j.issn.1673-548X.2017.07.007 |
| 摘要点击次数: 1367 |
| 全文下载次数: 1113 |
|
| 基金项目:国家自然科学基金资助项目（81260291） |
|
| 中文摘要:目的 探讨A型肉毒毒素对体外培养的人增生性瘢痕成纤维细胞增殖、凋亡及对ERK/MAPK信号通路的影响。方法 采用组织块法将人增生性瘢痕成纤维细胞分离并培养。将其分为对照组及A型肉毒毒素组。A型肉毒毒素组则给予含有0.4U/L A型肉毒毒素的DMEM培养基进行培养。对照组采用单纯DMEM培养基培养。在第1、3、5、7天时采用CCK8试剂盒检测细胞增殖并比较。采用流式细胞术对对照组和A型肉毒毒素干预的成纤维细胞行凋亡检测。Western blot法检测对照组、A型肉毒毒素组及U0126干预组成纤维细胞Ⅰ型胶原蛋白（collagen Ⅰ）， p-ERK1/2及总ERK蛋白相对表达量。结果 A型肉毒毒素能够显著抑制成纤维细胞增殖活性，干预7天时，细胞数量仅为对照组的68.9%。A型肉毒毒素组成纤维细胞的凋亡比率为35.9%，显著高于对照组。三组成纤维细胞的总ERK1/2蛋白相对表达量比较差异无统计学意义（P>0.05），而p-ERK1/2在对照组则仍显著高表达，但A型肉毒毒素组和U0126组的p-EKR1/2则显著受抑制，且低表达。Ⅰ型胶原蛋白则在对照组中高表达，显著高于A型肉毒毒素组和U0126组（P<0.05）。结论 A型肉毒毒素能够通过抑制ERK/MAPK信号通路而使得成纤维细胞凋亡增加，增殖活性减弱，Ⅰ型胶原蛋白分泌减少。 |
| 中文关键词:A型肉毒毒素 增生性瘢痕 成纤维细胞 ERK MAPK |
| |
| Effects of Botulinum Toxin Type A on Proliferation, Apoptosis and ERK/MAPK Signaling Pathway of Hypertrophic Scar Fibroblasts |
|
|
| Abstract:Objective To investigate the effects of botulinum toxin type A on the proliferation, apoptosis and ERK/MAPK signaling pathway of human hypertrophic scar fibroblasts in vitro. Methods Fibroblasts were isolated from human hypertrophic scar and cultured by tissue explant method. The cells were divided into control and botulinum toxin type A groups. The botulinum toxin group A was cultured in DMEM medium containing 0.4U/L botulinum toxin type A. The control group was cultured with DMEM alone. Cell proliferation was measured by CCK8 kit at days 1, 3, 5 and 7 and compared within groups. Apoptosis of fibroblasts was detected by flow cytometry which treated with botulinum toxin and control group. Western blot was used to detect the relative expression of collagen Ⅰ, p-ERK1/2 and total ERK protein in fibroblasts of control, botulinum toxin type A and U0126 groups. Results Botulinum toxin type A could significantly inhibit the proliferation of fibroblasts, the number of cells was only 68.9% in the control group at 7th days. The apoptotic rate of botulinum toxin type A was 35.9%, which was significantly higher than that of the control group. ERK 1/2 protein expression in the three groups was not significantly different (P> 0.05), while p-ERK 1/2 expression in the control group was still significantly higher, but the p-EKR 1/2 in botulinum toxin type A and U0126 group was significantly inhibited. Collagen Ⅰ was highly expressed in the control group, which was significantly higher than that in the botulinum toxin type A and U0126 groups (P<0.05). Conclusion Botulinum toxin type A can inhibit the ERK/MAPK signaling pathway and increased fibroblasts apoptosis, decreased proliferation activity and collagen Ⅰ secretion. |
| keywords:Botulinum toxin type A Hypertrophic scar Fibroblast ERK MAPK |
| 查看全文 查看/发表评论 下载PDF阅读器 |
