| CD19+ CD24high CD27+调节性B细胞在儿童传染性单核细胞增多症中的表达及意义 |
| 投稿时间：2016-11-07 修订日期：2016-12-08 点此下载全文 |
| 引用本文：王跃飞,陈葆国,章鸯,郑瑞.CD19+ CD24high CD27+调节性B细胞在儿童传染性单核细胞增多症中的表达及意义[J].医学研究杂志,2017,46(8):106-109,131 |
| DOI：
10.11969/j.issn.1673-548X.2017.08.027 |
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| 基金项目:浙江省151人才工程基金资助项目[台人社发（2011）121号] |
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| 中文摘要:目的 探讨调节性B细胞（Breg）在儿童传染性单核细胞增多症（IM）中的作用。方法 应用流式细胞术检测53例IM患儿外周血T淋巴细胞亚群，CD19+ CD24high CD27+ Breg细胞表达情况。用荧光定量PCR检测血浆EBV-DNA。同时用ELISA方法分析血清IL-10、TGF-β分泌水平。以50例健康儿童为对照。结果 IM急性期组与对照组比较CD3+ CD4+ T细胞明显降低（t=19.99，P<0.01），CD3+ CD8+ T细胞明显增高（t=17.47，P<0.01），CD4/CD8比值降低（t=16.79，P<0.01）。IM恢复期组与急性期组比较，CD3+ CD4+ T细胞明显增高（t=21.54，P<0.01），CD3+ CD8+ T细胞明显降低（t=17.47，P<0.01），CD4/CD8比值增高（t=16.68，P<0.01）。IM急性期组CD19+ CD24high CD27+ Breg细胞表达水平13.4%±1.87%，与对照组比较显著降低，差异有统计学意义（t=14.61，P<0.01）。IM急性期组CD19+ CD24high CD27+ Breg细胞表达水平与EBV-DNA病毒载量呈负相关（r=-0.52，P<0.01）。经治疗后IM恢复期组CD19+ CD24high CD27+ Breg细胞表达水平18.3%±2.11%与IM急性期组比较显著增高，差异有统计学意义（t=12.61，P<0.01）。IM急性期组血清IL-10较对照组明显降低，差异有统计学意义（t=11.50，P<0.01）。治疗后IM恢复期组IL-10较IM急性期组明显增高，差异有统计学意义（t=9.40，P<0.01）。结论 IM急性期存在明显免疫失衡，即CD3+ CD8+ T细胞增多，CD4/CD8比值降低，可能与Breg细胞表达减低导致的免疫抑制功能不足有关。 |
| 中文关键词:CD19+ CD24high CD27+调节性B细胞 IL-10 传染性单核细胞增多症 儿童 |
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| Role of CD19+ CD24high CD27+ Regulatory B Cells in Children with Infectious Mononucleosis |
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| Abstract:Objective To investigate the biological function of regulatory B cells (Bregs) in children with infectious mononucleosis (IM). Methods A total of 53 IM children and 50 healthy controls were enrolled.The T-cell subset and expressions of CD19+ CD24high CD27+ Bregs in peripheral blood mononuclear cells (PBMC) were evaluated by flow cytometry. The level of serum EBV-DNA copies was detected with fluorescent quantitative PCR. And the serum level of IL-10,TGF-β was measured by enzyme-1inked immunosorbent assay (ELISA). Results IM patients in acute phase group had a significantly higher percentage of CD3+ CD4+ T cells(t=19.99,P<0.01) and lower percentage of CD3+ CD8+ T (t=17.47,P<0.01) cells than control,corresponding to a low ratio of CD4/CD8(t=16.79,P<0.01).Compared with IM children in acute phase, the IM children in recovery phase had a higher expression of CD3+ CD4+ T cells(t=21.54,P<0.01) and lower expression of CD3+ CD8+ T cells(t=17.47,P<0.01), and higher ratio of CD4/CD8(t=16.68,P<0.01). The percentage of CD19+ CD24high CD27+ Breg expression in PBMC of IM children in acute phase was 13.4%±1.87%, and it was lower than those in the health control group(t=14.61,P<0.01). The expression level of CD19+ CD24high CD27+ Breg cells in the acute phase of IM was negatively correlated with EBV-DNA viral load (r=-0.52, P<0.01). The percentage of CD19+ CD24high CD27+ Breg expression in PBMC of post-treatment IM children in recovery phase was 18.3%±2.11%, and it was higher than those in the IM children in acute phase(t=12.61,P<0.01).The level of IL-10 significantly decreased in serum of IM patients in acute phase compared with control(t=11.50,P<0.01). The level of IL-10 significantly increased in serum of IM patients in recovery phase compared with IM patients in acute phase(t=9.40,P<0.01). Conclusion In the acute phase of IM, there was obvious immune imbalance, that is, the increase of CD3+ CD8+ T cells and the decrease of CD4/CD8 ratio, which may be result from the decreased level of Breg cells and its insufficient of immunosuppression function. |
| keywords:CD19+ CD24high CD27+ Bregs IL-10 Infectious mononucleosis Children |
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