| ID-LC-MS/MS评估糖尿病大鼠DNA和RNA氧化损伤 |
| 投稿时间：2017-04-09 修订日期：2017-04-24 点此下载全文 |
| 引用本文：王晚霞,罗顺斌,夏梦明,胡国新,蔡剑平.ID-LC-MS/MS评估糖尿病大鼠DNA和RNA氧化损伤[J].医学研究杂志,2017,46(12):19-24 |
| DOI：
10.11969/j.issn.1673-548X.2017.12.006 |
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| 基金项目:国家自然科学基金资助项目（81171028，81571058） |
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| 中文摘要:目的 评估高血糖对DNA和RNA氧化损伤的影响。方法 以链脲佐菌素诱导的糖尿病大鼠为模型，运用放射性核素稀释高效液相-串联质谱（ID-LC-MS/MS）法检测大鼠肾性、心性、肝性、肺、脑、睾丸、附睾、血浆和尿中的核酸氧化标志物8-氧化脱氧鸟苷（8-oxo-dGsn）和8-氧化鸟苷（8-oxo-Gsn）的含量，并进行比较分析。结果 虽然不同脏器DNA中8-oxo-dGsn/106 dGsn和RNA中8-oxo-Gsn/106 Gsn的基础水平不一致，但是模型组SD大鼠不同脏器中8-oxo-Gsn/106 Gsn和8-oxo-dGsn/106 dGsn的水平均高于对照组，6月组糖尿病大鼠肾脏、肺脏和睾丸中8-oxo-dGsn/106 dGsn的水平高于对照组相应脏器的水平（P<0.05）；3月组糖尿病大鼠肾脏、心脏和睾丸中8-oxo-Gsn/106 Gsn的水平高于对照组相应脏器的水平（P<0.05）；除了脑和肺脏，6月组糖尿病大鼠各脏器中8-oxo-Gsn/106 Gsn的水平均高于其对照组（P<0.05）；糖尿病大鼠血浆8-oxo-dGsn和8-oxo-Gsn浓度明显高于其同龄对照大鼠，且随着高糖血症的持续而增加。尿液检测结果的趋势与血浆中的检测结果一致，RNA氧化代谢产物8-oxo-Gsn均高于DNA的氧化代谢产物8-oxo-dGsn。结论 高血糖引起的氧化应激增强可以造成多器官DNA和RNA氧化损伤增加，DNA和RNA氧化损伤随着高糖血症的持续而增加；RNA氧化损伤水平高于DNA氧化损伤，其标志物尿液8-oxo-Gsn可以作为评估糖尿病氧化应激状态的良好指标。 |
| 中文关键词:8-氧化鸟苷 8-氧化脱氧鸟苷 RNA氧化 糖尿病 氧化应激 |
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| Evaluation of DNA and RNA Oxidation in Hyperglycemic SD Rats Using ID-LC-MS/MS |
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| Abstract:Objective To evaluate DNA and RNA oxidation in diabetes mellitus.Methods We used a sensitive and accurate method based on isotope dilution high-performance liquid chromatography-triple quadruple mass spectrometry (ID-LC-MS/MS) to determine the levels of 8-oxo-7,8-dihydro-2-deoxyguanosine (8-oxo-dGsn) and 8-oxo-7,8-dihydroguanosin (8-oxo-Gsn) in various tissue specimens, plasma and urine of hyperglycemic SD rats induced by streptozotocin (STZ).Results The oxidative DNA and RNA damage were observed in various organs and the amounts of 8-oxo-dGsn and 8-oxo-Gsn derived from DNA and RNA were increased with hyperglycemic status. The levels of 8-oxo-Gsn in urine and plasma were significantly higher compared to that of 8-oxo-dGsn, which most likely reflected the RNA damage that occurs more frequently compared to DNA damage.Conclusion For the oxidative stress induced by hyperglycemia, 8-oxo-Gsn in urine may be a sensitive biomarker based on the results in urine, plasma and tissues. |
| keywords:8-oxo-7,8-dihydroguanosine 8-oxo-7,8-dihydro-2-deoxyguanosine RNA oxidation Diabetes mellitus Oxidative stress |
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