| ALDH1高表达对乳腺癌细胞MCF-7干细胞特性的影响 |
| 投稿时间：2017-08-13 修订日期：2017-09-20 点此下载全文 |
| 引用本文：冀峰,李克.ALDH1高表达对乳腺癌细胞MCF-7干细胞特性的影响[J].医学研究杂志,2018,47(6):68-71 |
| DOI：
10.11969/j.issn.1673-548X.2018.06.016 |
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| 基金项目:河南省科技厅基础与前沿项目（112300410044） |
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| 中文摘要:目的 探讨ALDH1高表达对乳腺癌MCF-7细胞干细胞特性的影响，为乳腺癌的治疗提供理论依据。方法 构建慢病毒ALDH1RNA，转染乳腺癌MCF-7细胞，嘌呤霉素筛选稳定高表达ALDH1的亚克隆，命名为MCF-ALDH1细胞株。荧光定量PCR和Western blot法检测MCF-ALDH1细胞株及对照细胞中ALDH1在mRNA和蛋白水平的表达；克隆形成实验、悬浮球形成实验及裸鼠成瘤实验检测MCF-ALDH1及对照组细胞的干细胞特性，MTT法测定细胞的耐药性。结果 与MCF-7组细胞比较，MCF-ALDH1细胞株的ALDH1在mRNA和蛋白表达量明显升高；MCF-ALDH1细胞克隆形成数目为128±22个，高于MCF-ALDH1细胞球克隆形成数（46±15个）；悬浮成球实验，MCF-ALDH1细胞悬浮成球数目明显增多（16±2个），高于对照细胞悬浮球数（8.0±1.5个）；肿瘤细胞在裸鼠皮下接种后，MCF-7细胞组有3只裸鼠成瘤，MCF-ALDH1有5只成瘤，瘤体积明显大于MCF-7组，差异有统计学意义（P<0.05）。耐药实验显示，MCF-ALDH1对顺铂、紫杉醇的耐药性明显提高。结论 高表达ALDH1的乳腺癌MCF-7细胞干细胞特性增强，ALDH1可能成为乳腺癌治疗的靶点之一。 |
| 中文关键词:ALDH1 乳腺癌 干细胞特性 |
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| Expression and Significance of ALDH1 in Cancer Stem Cell-like Characteristics in Breast Cancer Cell Line MCF-7 |
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| Abstract:Objective To investigate the expression and significance of ALDH1 in cancer stem cell-like characteristics in breast cancer cell line MCF-7. Methods RNA lenti-virus particles targeting ALDH1 gene were transfected into MCF-7 cells. Subclones with stable ALDH1 expression were selected by puromycin and named MCF-ALDH1 cells. The mRNA and protein expression of ALDH1 were detected by real-time quantitative PCR and Western blot tests. Cell differentiation,stem cell potential were observed by clone formation assay and suspension sphere-forming assay. The abilities of tumor-formation of cells were detected through inoculating the cells into the nod mouse. To detect the drug-resistance ability of cells by MTT test. Results Compared to the MCF-7 cells, the expression of mRNA and protein of ALDH1 by MCF-ALDH1 cells were significantly increased. Clony forming numbers(128±22) of the MCF-ALDH1 cells was higher than controls(46±15)(P<0.05), The proportion of sphere-initiating cells in MCF-ALDH1 and controls was (16±2)versus(8.0±1.5, P<0.05). The average tumor volume of MCF-ALDH1 was greater than controls(P<0.05).The drug resistance index of MCF-ALDH1 was higher than controls. Conclusion Experimental findings from this study showed that MCF-7 cells highly expressing ALDH1 genes, with higher proliferation and differentiation, in vivo tumorigenicity and potential of stem cells. ALDH1 may be used as an target to cure breast cancer. |
| keywords:ALDH1 Breast cancer Stem cell-like characteristics |
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