| NF-κB对病毒性心肌炎小鼠心肌中促炎细胞因子的调控 |
| 投稿时间：2017-10-26 修订日期：2017-11-21 点此下载全文 |
| 引用本文：薛陆静,潘海燕.NF-κB对病毒性心肌炎小鼠心肌中促炎细胞因子的调控[J].医学研究杂志,2018,47(8):45-47,53 |
| DOI：
10.11969/j.issn.1673-548X.2018.08.011 |
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| 基金项目:国家自然科学基金青年科学基金资助项目（81100154） |
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| 中文摘要:目的 探讨核因子-κB （NF-κB）对A型流感病毒（IAV）性心肌炎小鼠心肌中促炎细胞因子的调控作用。方法 30只8周龄雄性BALB/c小鼠随机分3组：1正常对照组（C组）：经鼻吸入15μl生理盐水；2感染对照组（I组）：经鼻吸入40空斑形成单位（pfu） IAV；3PDTC组（P组）：经鼻吸入40pfu IAV，腹腔注射NF-κB抑制剂吡咯烷二硫代氨基甲酸（PDTC）（10mg/kg），每天1次。感染后第9天处死小鼠，切取心脏组织进行病理及生化检查。结果 IAV感染可诱导心肌中促炎细胞因子白介素（IL）-1β、IL-6及肿瘤坏死因子（TNF）α显著上调（P<0.01，P<0.05），引发心肌急性炎性反应，部分心肌组织坏死。PDTC显著抑制促炎细胞因子在心肌中表达（P<0.01），有效抑制IAV病毒复制（P<0.01），减轻心肌炎性反应。结论 IAV感染心肌组织后可能通过NF-κB信号通路诱导心肌中促炎细胞因子表达上调，抑制NF-κB激活可能为预防和治疗流感病毒性心肌炎提供新的思路。 |
| 中文关键词:A型流感病毒 病毒性心肌炎 核因子-κB 促炎细胞因子 |
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| Regulation of Proinflammatory Cytokine Expression by NF-κB in Viral Myocarditis |
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| Abstract:Objective To investigate the regulatory effects of nuclear factor (NF)-κB on the expression of proinflammatory cytokines in influenza A virus (IAV)-induced myocarditis. Methods Thirty male BALB/c mice of 8 weeks old were randomly divided into 3 groups:1Normal control group (group C):15μL saline with nasal inhalation;2Infection control group (group I):infected intranasally with 40 plaque formation unit (pfu) IAV;3PDTC group (group P):infected intranasally with 40 pfu IAV and injected intraperitoneally with NF-κB inhibitor pyrrolidine dithiocarbamic acid (PDTC)(10mg/kg), once daily. The mice were euthanized at 9 days after infection and the heart tissue was taken for pathological and biochemical examination. Results IAV infection induced significant up-regulation of proinflammatory cytokines (IL) -1β,IL-6 and tumor necrosis factor(TNF) α in myocardium (P<0.01, P<0.05), and triggered acute myocarditis, and some myocardial necrosis. PDTC significantly inhibited the expression of proinflammatory cytokines(P<0.01), and effectively suppressed the IAV replication(P<0.01), and myocardial inflammatory response. Conclusion IAV infection induces up-regulation of proinflammatory cytokines in myocardium might through the NF-κB signaling pathway inhibiting activation of NF-κB may provide new thinking for the prevention and treatment of influenza induced myocarditis. |
| keywords:Influenza A virus Viral myocarditis Nuclear factor-κB Pro-inflammatory cytokines |
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