| Coronin3促进食管癌Eca-109细胞的迁移和侵袭 |
| 投稿时间：2017-12-21 修订日期：2018-01-04 点此下载全文 |
| 引用本文：林宇,陈俊强,周智峰,何诚,郑飞,刘萍萍,陈元美,李建成,朱坤寿.Coronin3促进食管癌Eca-109细胞的迁移和侵袭[J].医学研究杂志,2018,47(9):67-72 |
| DOI：
10.11969/j.issn.1673-548X.2018.09.016 |
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| 基金项目:福建省自然科学基金资助项目（2015J01377）；福建省科技厅重点项目（2011Y0014）；福建省临床重点专科建设项目（2012） |
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| 中文摘要:目的 探讨Coronin3基因促进食管癌Eca-109细胞株迁移和侵袭的可能机制。方法 用慢病毒稳转的方式构建过表达和敲减Coronin3的Eca-109食管癌细胞株，通过划痕和Transwell实验分别检测Coronin3基因对Eca-109食管癌细胞的迁移和侵袭的影响。Western blot法实验检测Coronin3对CDH11及E-cadherin、N-cadherin等EMT标志分子蛋白表达的影响。实时荧光定量PCR实验检测Coronin3对CDH11及E-cadherin、N-cadherin等EMT标志分子mRNA表达的影响。结果 笔者成功构建了Coronin3过表达和敲减的Eca-109细胞株。Coronin3过表达能够促进细胞迁移和侵袭。与之相反，Coronin3敲减能够显著抑制细胞迁移和侵袭。Coronin3过表达能够提高CDH11的蛋白和mRNA水平，提高α-SMA、N-cadherin的蛋白和mRNA水平，降低E-cadherin的蛋白和mRNA水平。与之相反，Coronin3敲减能显著降低CDH11的蛋白和mRNA水平，降低α-SMA、N-cadherin的蛋白和mRNA水平，提高E-cadherin的蛋白和mRNA水平。结论 Coronin3可以通过促进上皮-间质转换（EMT）来调控食管癌的转移和侵袭，为治疗食管癌提供了潜在的新靶标。 |
| 中文关键词:Coronin3 食管癌 转移 侵袭 上皮-间质转换 |
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| Impact of Coronin3 Gene on Migration and Invasion of Esophagus Carcinoma Cell Line Eca-109 |
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| Abstract:Objective To study the mechanism of coronin3 promote esophagus carcinoma cell line Eca-109 migration and invasion. Methods We established Coronin3 overexpression and Coronin3 knockdown stable Eca-109 cell lines through lentvirus infection. Then we used wound healing and Transwell assays to detect the ability of tumor migration and invasion. Western blot and real-time PCR assays validated the protein and mRNA level of CDH11, E-cadherin,N-cadherin, α-SMA up on Coronin3 over-expression and knockdown. Results We successfully established Coronin3 over-expression and Coronin3 knockdown stable Eca-109 cell lines. Coronin3 over-expression promoted Eca-109 cell migration and invasion. Conversely, Coronin3 knockdown reversed the progress. Coronin3 over-expression up-regulated the protein and mRNA level of CDH11, α-SMA,N-cadherin, down-regulated the protein and mRNA level of E-cadherin, and vice versa. Conclusion Coronin3 might regulate esophagus cancer migration and invasion through epithelial-mesenchymal transitions progression. Thus we suggest that Coronin3 might be a novel therapeutic target for esophagus cancer treatment. |
| keywords:Coronin3 Esophagus cancer Migration Invasion Epithelial-mesenchymal transitions |
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