| miRNA-467b在痛性糖尿病神经病变中对TNF-α的调节作用 |
| 投稿时间：2017-12-06 修订日期：2017-12-25 点此下载全文 |
| 引用本文：夏志强,郝利军,吴斌.miRNA-467b在痛性糖尿病神经病变中对TNF-α的调节作用[J].医学研究杂志,2018,47(10):19-22 |
| DOI：
10.11969/j.issn.1673-548X.2018.10.007 |
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| 基金项目:国家自然科学基金资助项目（30871283） |
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| 中文摘要:目的 探讨miRNA-467b在痛性糖尿病神经病变中对TNF-α的调节作用。方法 SD大鼠腹腔内注射链脲佐菌素建立痛性糖尿病神经病变（模型组，n=20），空白组（n=20）注射生理盐水；模型组在建模完成后1周分为两组-实验组与对照组，每组10只大鼠，实验组注射人工合成的miRNA-467b，对照组注射等量的生理盐水，检测所有大鼠神经痛性状况与miRNA-467b、TNF-α表达水平。结果 模型组大鼠的机械痛和热痛的反应潜伏期相比空白组都减少（P<0.05），并且实验组的机械痛和热痛的反应潜伏期也高于对照组（P<0.05）。模型组大鼠的miRNA-467b表达量相比空白组减少，TNF-α表达量上升，差异有统计学意义（P<0.05）；并且实验组的miRNA-467b、TNF-α mRNA表达水平与对照组比较差异有统计学意义（P<0.05）。实验组大鼠的TNF-α蛋白表达量下降，与对照组比较差异有统计学意义（P<0.05）；同时模型组的TNF-α蛋白表达量高于空白组（P<0.05）。结论 痛性糖尿病神经病变中miRNA-467b呈现下调表达状况，可导致TNF-α表达上升，从而引起机械痛和热痛的产生。 |
| 中文关键词:机械痛 热痛 miRNA-467b 糖尿病神经病变 TNF-α |
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| Regulation of miRNA-467b on TNF-α in Painful Diabetic Neuropathy |
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| Abstract:Objective To investigate the effect of miRNA-467b on the regulation of TNF-α in painful diabetic neuropathy. Methods SD rats by intraperitoneal injection of streptozotocin painful diabetic neuropathy (model group, n=20), theblank group (n=20) were injected of saline. Model group in 1 weeks after modeling were divided into two groups:experimental group and control group, 10 rats in each group. The experimental group were given the artificial injection the synthesis of miRNA-467b, and the control group were injected with normal saline. All the rats were to detect neuralgic condition and miRNA-467b, TNF-α expression level. Results The latency of mechanical pain and thermal pain in the model group were lower than that in the blank group (P<0.05), and the latency of mechanical pain and thermal pain in the experimental group was also higher than that in the control group (P<0.05). The rats in the model group the expression of miRNA-467b compared with the blank group were lower, and the TNF-α expression were increased, there were statistically significant difference (P<0.05). And the miRNA-467b, TNF-α mRNA expression level in the experimental group were statistically significant compared with the control group (P<0.05). The expression of TNF-α protein in the experimental group was decreased, compared with the control group were statistically significant difference(P<0.05), and the expression of TNF-α protein in the model group was higher than that in the blank group (P<0.05). Conclusion The expression of miRNA-467b is down-regulated in painful diabetic neuropathy that can induces the expression of TNF-α and cause mechanical pain and thermal pain. |
| keywords:Mechanical pain Heat pain miRNA-467b Diabetic neuropathy TNF- alpha |
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