| TUG1对氧糖剥夺/复氧损伤后人脐静脉内皮细胞生物学行为的影响 |
| 投稿时间：2024-03-25 修订日期：2024-03-27 点此下载全文 |
| 引用本文：李斐,张新元,江洪祥,张惠凯,陈谦学.TUG1对氧糖剥夺/复氧损伤后人脐静脉内皮细胞生物学行为的影响[J].医学研究杂志,2025,54(1):31-36 |
| DOI：
10.11969/j.issn.1673-548X.2025.01.007 |
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| 基金项目:国家自然科学基金资助项目(面上项目)(82071299) |
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| 中文摘要:目的 探讨牛磺酸上调基因1(taurine-upregulated gene 1,TUG1)对人脐静脉内皮细胞(human umbilical vein endothelial cell, HUVEC)在氧糖剥夺/复氧(oxygen-glucose deprivation/reoxygenation,OGD/R)损伤后凋亡、增殖、迁移等生物学功能的影响。方法 OGD/R处理HUVEC建立OGD/R损伤的细胞模型,分别转染沉默TUG1的小干扰RNA(small interfering RNA-TUG1, si-TUG1, si-TUG1)及其空白对照(si-NC),将细胞分为对照组,OGD/R组、OGD/R+si-NC组和OGD/R+si-TUG1组,PCR检测细胞TUG1的表达水平,流式细胞术检测各组细胞凋亡,CCK-8实验检测细胞增殖,划痕实验检测细胞迁移,免疫荧光检测细胞中CD31表达,Western blot法检测细胞中核增殖抗原(proliferating cell nuclear antigen,PCNA)蛋白的表达。结果 PCR结果显示,OGD/R组细胞中TUG1的表达水平显著高于对照组(P＜0.05)；转染si-TUG1可显著降低HUVEC中TUG1的表达水平(P＜0.05)；si-TUG1组HUVEC的凋亡率显著低于si-NC组(P＜0.05)；si-TUG1组HUVEC的增殖、迁移、CD31免疫荧光强度以及PCNA蛋白表达水平均高于si-NC组(P＜0.05)。结论 降低TUG1的表达则可显著减少OGD/R条件下HUVEC的凋亡,增加其增殖、迁移、成管能力和细胞活性。 |
| 中文关键词:牛磺酸上调基因1 人脐静脉内皮细胞 凋亡 增殖 迁移 |
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| Effect of TUG1 on the Biological Behavior of Human Umbilical Vein Endothelial Cells after Oxygen Glucose Deprivation/Reoxygenation Injury. |
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| Abstract:Objective To investigate the effects of taurine-upregulated gene 1 (TUG1) on the biological functions of human umbilical vein endothelial cells (HUVEC) such as apoptosis, proliferation, and migration after oxygen-glucose deprivation/reoxygenation (OGD/R) injury. Methods HUVEC were treated with OGD/R to establish a cell model injured by OGD/R. Small interfering RNA silencing TUG1 (si-TUG1) and its negative control (si-NC) were transfected, and the cells were divided into the control group, OGD/R group, OGD/R+si-NC group, and OGD/R+si-TUG1group. PCR was used to detect the expression level of TUG1 in HUVEC; flow cytometry was used to detect cell apoptosis; CCK-8 assay was used to detect cell proliferation; wound-healing assay was used to detect cell migration; immunofluorescence was used to detect the expression of CD31 in HUVEC; and Western blot was used to detect the expression of nuclear proliferation antigen (PCNA) protein. Results The PCR results showed that the expression level of TUG1 in OGD/R group cells was significantly higher than that in the control group (P<0.05); si-TUG1 transfection can significantly reduce the expression level of TUG1 in HUVEC (P<0.05); the apoptosis rate of HUVEC in the si-TUG1group was significantly lower than that in the si-NC group (P<0.05); The cell proliferation, cell migration, immunofluorescence intensity of CD31, and the expression levels of PCNA protein in the si-TUG1group were higher than those in the si-NC group (P<0.05). Conclusion Inhibiting TUG1 can significantly reduce the apoptosis of HUVEC under OGD/R conditions, and increase the biological functions of HUVEC, such as proliferation, migration, and tubular ability. |
| keywords:Taurine-upregulates gene 1 Human umbilical vein endothelial cells Apoptosis Proliferation Transfer |
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