| 电刺激对压力性损伤大鼠感染创面愈合的作用 |
| 投稿时间：2024-08-13 修订日期：2024-09-19 点此下载全文 |
| 引用本文：袁邻雁,吴孟航,杨雪,刘晓艳,李玲利.电刺激对压力性损伤大鼠感染创面愈合的作用[J].医学研究杂志,2025,54(2):43-47, 87 |
| DOI：
10.11969/j.issn.1673-548X.2025.02.008 |
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| 基金项目:国家自然科学基金资助项目(面上项目)(52373237)；四川省自然科学基金资助项目(面上项目)(2023NSFSC0541)；四川大学华西护理学科发展基金资助项目(HXHL21005) |
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| 中文摘要:目的 探讨电刺激治疗大鼠压力性损伤感染创面的愈合效果。方法 采用随机数字表法将24只雄性SD大鼠分为空白组、模型组和电刺激组。采用磁铁外加压方法建立3期压力性损伤感染创面模型并观察2周。模型组自然愈合,电刺激组采用电针进行电刺激。分别于造模后第1、3、5、7、10、14天测量大鼠创面面积,计算创面愈合率,评估第3、7、14天的实验动物创面评估工具(experimental wound assessment tool,EWAT)评分。在第7、14天,采用苏木精-伊红染色及Masson染色观察创面皮肤组织病理变化,通过荧光染色测定诱导型一氧化氮合酶(inducible nitric oxide synthase,INOS)、CD206、CD31、α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)抗体的表达水平。结果 在治疗第3、5、7、10、14天,电刺激组创面愈合率高于模型组,差异有统计学意义(P＜0.05)；第3、7、14天的创面EWAT评分低于模型组,差异有统计学意义(P＜0.05)。组织形态观察显示,电刺激可以减轻创面炎症反应,促进毛细血管生成及胶原沉积,加速上皮爬行。与模型组比较,电刺激治疗可以降低INOS抗体表达水平,提高CD206抗体、α-SMA抗体和CD31抗体表达水平,差异有统计学意义(P＜0.05)。结论 电刺激可以有效促进3期压力性损伤大鼠感染创面愈合,促进巨噬细胞极化及血管重建。 |
| 中文关键词:电刺激 压力性损伤 压疮 感染创面 大鼠 |
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| Effect of Electrical Stimulation on Healing of Infected Wound in Rats with Infected Pressure Injury. |
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| Abstract:Objective To investigate the healing effect of electrical stimulation in the treatment of infected pressure injury in rats. Methods Twenty-four male SD rats were divided into blank group, model group and electrical stimulation group by random number table method. The infected pressure injury model of stage 3 was established by magnet external compression and observed for 2 weeks. The model group healed naturally, and the electrical stimulation group underwent electroacupuncture. The wound areas of rats were measured on the 1st, 3rd, 5th, 7th, 10th and 14th days after modeling, the wound healing rate was calculated, and the experimental wound assessment tool (EWAT) scores on the 3rd, 7th and 14th days were evaluated. On the 7th and 14th days, hematoxylin-eosin staining and Masson staining were used to observe the pathological changes of wound skin tissue, and the expression levels of inducible nitric oxide synthase (INOS), CD206, CD31 andα-smooth muscle actin (α-SMA) antibodies were measured by fluorescent staining. Results The wound healing rate of the electrical stimulation group were higher than that in the model group at the 3rd, 5th, 7th, 10th and 14th days of treatment, and the difference were statistically significant (P<0.05). The EWAT scores of wounds in the electrical stimulation group were lower than that in the model group at the 3rd, 7th and 14th days, and the difference were statistically significant (P<0.05). Tissue morphology observation showed that electrical stimulation reduced wound inflammation, promoted capillary production and collagen deposition, and accelerated epithelial crawling. Compared with the model group, electrical stimulation treatment reduced the expression level of INOS antibody and increased the expression levels of CD206 antibody, α-SMA antibody and CD31 antibody, and the difference was statistically significant (P<0.05). Conclusion Electrical stimulation can effectively promote the healing of infected wounds in rats with stage 3 pressure injury, promotes macrophage polarization and vascular reconstruction. |
| keywords:Electrical stimulation Pressure injury Pressure sore Infected wound Rats |
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