| 基于孟德尔随机化方法探析中医“因虚致痫”理论的科学内涵 |
| 投稿时间：2024-08-27 修订日期：2024-10-11 点此下载全文 |
| 引用本文：陈俊龙,刘佳霖,郑香春,李淑芳,许晨曌,王苏妹.基于孟德尔随机化方法探析中医“因虚致痫”理论的科学内涵[J].医学研究杂志,2025,54(2):96-102 |
| DOI：
10.11969/j.issn.1673-548X.2025.02.016 |
| 摘要点击次数: 234 |
| 全文下载次数: 68 |
|
| 基金项目:国家自然科学基金资助项目(81774277)；全国第七批老中医学术继承工作项目{国中医药办人教发〔2021〕272号}；教育部产学合作协同育人项目(230809024907236)；北京中医药大学重点攻关项目(2020-JYB-ZDGG-131)；北京中医药大学基本科研业务项目(2020-tsxk-001) |
|
| 中文摘要:目的 基于中医“因虚致痫”理论,采用双样本孟德尔随机化方法探讨虚弱与癫痫之间的因果关系,以期丰富“因虚致痫”理论的现代内涵,并为中医从虚论治癫痫提供理论依据。方法 笔者从公开的全基因组关联的汇总数据集中提取虚弱与癫痫的数据,通过设置条件(以P<5×10-8为筛选条件,连锁不平衡系数设定为0.001,连锁不平衡区域宽度为10000kb),筛选出最终作为工具变量的单核苷酸多态性(single nucleotide polymorphism, SNP)。应用MR-Egger回归法、加权中位数法、逆方差加权法、简单模式和加权模式5种方法进行孟德尔随机化分析,以逆方差加权法作为主要分析方法,并采用OR值及95% CI对虚弱与癫痫之间的因果关系进行解释。多效性检验采用MR-Egger截距和MR-PRESSO分析方法,异质性分析采用MR-Egger的Cochran′s Q检验,同时采用“留一法”检验进行敏感度分析。结果 逆方差加权法和加权中位数法分析结果显示,虚弱与癫痫之间存在正向因果关系,该结果通过了多效性检验,证明结果具有稳健性。结论 虚弱与癫痫发生风险升高相关,这与历代医家“因虚致痫”的认识一致。提示在临床治疗中,除了常规化痰祛瘀、熄风开窍外,更应注重补虚培元。 |
| 中文关键词:癫痫 虚弱 孟德尔随机化 “因虚致痫”理论 中医学 |
| |
| Scientific Connotations of the Traditional Chinese Medicine Theory of "Epilepsy Caused by Frailty" Based on Mendelian Randomization. |
|
|
| Abstract:Objective To investigate the causal relationship between frailty and epilepsy based on the traditional Chinese medicine (TCM) theory of "epilepsy caused by frailty" utilizing a two-sample Mendelian randomization approach, to enrich the modern connotation of the "epilepsy caused by frailty" theory and provide a theoretical basis for treating epilepsy from a frailty perspective in TCM. Methods Data on frailty and epilepsy were extracted from publicly available genome-wide association study summary datasets. Single nucleotide polymorphism (SNP) were selected as instrumental variable under the conditions of P<5×10-8, a linkage disequilibrium coefficient of 0.001, and a linkage disequilibrium region width of 10000kb. Mendelian randomization analysis was performed using five methods:MR-Egger regression, weighted median, inverse variance weighting, simple mode and weighted mode, with inverse variance weighting as the primary analysis method. The causal relationship between frailty and epilepsy was interpreted using OR and 95% CI. Pleiotropy was assessed using the MR-Egger intercept and MR-PRESSO analysis methods, while heterogeneity was analyzed using MR-Egger Cochran′s Q test, and sensitivity analysis was conducted using the leave-one-out method. Results The results of inverse variance weighting and weighted median analysis indicated that there was a positive causal relationship between frailty and epilepsy. This result passed pleiotropy tests, confirming its robustness. Conclusion Frailty is associated with an increased risk of epilepsy, which is consistent with the TCM understanding of "epilepsy caused by frailty". It is suggested that in clinical treatment, besides conventional approaches such as resolving phlegm, eliminating blood stasis, calming wind, and opening orifices, there should be a greater emphasis on tonifying frailty and nurturing the body′s vital energy. |
| keywords:Epilepsy Frailty Mendelian randomization "Epilepsy caused by frailty" theory Traditional Chinese medicine |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
