| 小豆蔻明对舌鳞状细胞癌CAL27细胞增殖、 迁移和侵袭的影响 |
| 投稿时间：2024-07-21 修订日期：2024-09-26 点此下载全文 |
| 引用本文：蒋文芮,林健辉,韩瑞,刘新伟,周美云,张靓,徐锦程.小豆蔻明对舌鳞状细胞癌CAL27细胞增殖、 迁移和侵袭的影响[J].医学研究杂志,2025,54(2):132-138 |
| DOI：
10.11969/j.issn.1673-548X.2025.02.021 |
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| 基金项目:安徽省教育厅重大项目(KJ2021ZD0088) |
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| 中文摘要:目的 探讨小豆蔻明对舌鳞状细胞癌CAL27细胞增殖、迁移、侵袭的影响,并阐明其可能的作用机制。方法 分别使用不同浓度的小豆蔻明处理舌鳞状细胞癌CAL27细胞,处理时间为24h和48h。CCK-8实验检测细胞的增殖,同时筛选最佳药物浓度及作用时间用于后续实验；克隆形成实验检测细胞增殖能力；划痕实验和Transwell实验分别检测细胞迁移和侵袭能力；Western blot法检测各组细胞中上皮间质转化(epithelial-mesenchymal transition, EMT)相关蛋白及磷脂酰肌醇-3激酶/蛋白激酶B(phosphatidylinositol 3-kinase/protein kinase B, PI3K/Akt)通路相关蛋白的表达水平。结果 在一定的浓度范围内,小豆蔻明以浓度依赖的方式抑制舌鳞状细胞癌CAL27细胞的增殖、迁移及侵袭的能力,且随着浓度及作用时间的增加,抑制效果越明显；此外,随着药物浓度的增加,E-cadherin的蛋白表达依次呈上调趋势,N-cadherin、Vimentin、PI3K下游的磷酸化效应分子(p-PI3K、p-Akt)的蛋白表达呈下降趋势,使用PI3K激动剂740Y-P处理后可部分逆转小豆蔻明的抗癌作用,差异均有统计学意义(P＜0.05,P＜0.01)。结论 小豆蔻明能够抑制舌鳞状细胞癌CAL27细胞的增殖、迁移、侵袭,其作用机制可能通过抑制PI3K/Akt信号通路进而调控EMT过程有关,这为舌鳞状细胞癌的治疗提供了新的潜在策略与药物靶点。 |
| 中文关键词:舌鳞状细胞癌 CAL27细胞 小豆蔻明 迁移 侵袭 上皮间质转化 PI3K/Akt信号通路 |
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| Effect of Cardamonin on Proliferation, Migration and Invasion of CAL27 Cells in Tongue Squamous Cell Carcinoma. |
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| Abstract:Objective To investigate the effects of Cardamonin on the proliferation, migration and invasion of CAL27 cells in tongue squamous cell carcinoma, and to elucidate its possible mechanism of action. Methods CAL27 cells were treated with different concentrations of Cardamonin for 24h and 48h respectively. CCK-8 assay was used to detect cell proliferation, and the optimal drug concentration and action time were screened for subsequent experiments. Clonal formation assay was used to detect cell proliferation. Scratch test and Transwell test were used to detecte the ability of cell migration and invasion. Western blot method was used to detecte the expression levels of EMT related protein and phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway related protein. Results Within a certain concentration, Cardamonin inhibited the proliferation, migration and invasion of tongue squamous cell carcinoma CAL27 cells in a concentration-dependent manner, and the inhibitory effect became more obvious with the increase of concentration and time in a certain range. In addition, with the increase of drug concentration, the protein expression of E-cadherin showed an upward trend, while the protein expression of N-cadherin, Vimentin and phosphorylated efferent molecules downstream of PI3K (p-PI3K and p-Akt) showed a downward trend. Treatment with PI3K agonist 740Y-P could partially reverse the anticancer effect of Cardamonin, and the differences were statistically significant (P < 0.05, P < 0.01). Conclusion Cardamonin can inhibit the proliferation, migration and invasion of CAL27 cells in tongue squamous cell carcinoma cells, and its mechanism may be related to the regulation of EMT process by inhibiting PI3K/Akt signaling pathway, which provides a new potential strategy and drug target for the treatment of tongue squamous cell carcinoma. |
| keywords:Tongue squamous cell carcinoma CAL27 cell Cardamonin Migration Invasion Epithelial-mesenchymal transition PI3K/Akt signaling pathway |
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