| sST2蛋白和NK细胞在创伤性脑损伤患者中的水平及相关性研究 |
| 投稿时间：2024-07-18 修订日期：2024-11-25 点此下载全文 |
| 引用本文：龚雪,吕宏祥,王赛男.sST2蛋白和NK细胞在创伤性脑损伤患者中的水平及相关性研究[J].医学研究杂志,2025,54(3):137-141 |
| DOI：
10.11969/j.issn.1673-548X.2025.03.025 |
| 摘要点击次数: 29 |
| 全文下载次数: 32 |
|
| 基金项目:南京医科大学康达学院科研发展基金资助项目(KD2023KYJJ247) |
|
| 中文摘要:目的 分析创伤性脑损伤(traumatic brain injury,TBI)患者中可溶性生长刺激表达基因2(soluble growth stimulation expressed gene 2,sST2)蛋白和自然杀伤细胞(natural killer cell,NK细胞)的水平及其相关性。方法 选取2023年1月~2024年5月南京医科大学附属江宁医院收治的147例TBI患者作为TBI组,其中轻度55例,中度57例,重度35例,另外选取30例健康体检者作为对照组。采用流式荧光法检测2组研究对象血清中细胞因子白细胞介素(interleukin,IL)-2、IL-6、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和IL-17A的含量,采用流式细胞术检测2组研究对象外周血中NK细胞(CD45+CD3-CD19-CD56+)的比例,并通过免疫荧光干式定量法检测2组研究对象血清中的sST2水平,进而探讨TBI患者血清sST2水平与NK细胞比例之间的相关性。结果 与对照组比较,所有TBI组患者的血清sST2表达水平均明显升高,细胞因子IL-2、IL-6、TNF-α和IL-17A的水平也明显升高,重度TBI患者NK细胞的比例则显著降低,差异均有统计学意义(P<0.05)。另外,中度和重度TBI患者血清sST2的表达水平与NK细胞的比例呈负相关(r=-0.328,P<0.01),而轻度TBI患者中这两项指标无显著相关性(P>0.05)。结论 中度和重度TBI患者sST2表达水平与NK细胞具有相关性,而轻度TBI患者sST2表达水平与NK细胞间无显著相关性。 |
| 中文关键词:可溶性生长刺激表达基因2蛋白 NK细胞 创伤性脑损伤 |
| |
| Level and Correlation of sST2 and NK Cell in Patients with Traumatic Brain Injury. |
|
|
| Abstract:Objective To analyze the levels of soluble growth stimulation expressed gene 2 (sST2) and natural killer (NK) cell in traumatic brain injury (TBI) patients and their correlation. Methods A total of 147 patients with TBI admitted to Affiliated Jiangning Hospital, Nanjing Medical University from January 2023 to May 2024 were selected as the TBI group, including 55 cases of mild, 57 cases of moderate, and 35 cases of severe. Additionally, 30healthy individuals were selected as the control group. Flow cytometry fluorescence assay was utilized to measure the levels of cytokines interleukin (IL)-2, IL-6, tumor necrosis factor-α (TNF-α), and IL-17A in the serum of both groups. Flow cytometry was also employed to determine the proportion of NK cell (CD45+CD3-CD19-CD56+) in the peripheral blood of the subjects. Furthermore, an immunofluorescence dry quantitative method was used to assess the levels of sST2 in the serum of the subjects, with the goal of investigating the association between serum sST2 levels and the proportion of NK cell in TBI patients. Results Compared with the control group, the expression levels of serum sST2 in TBI group were significantly increased, and the levels of cytokines IL-2, IL-6, TNF-α and IL-17A were also significantly increased. The proportion of NK cell was significantly decreased in severe TBI patients, the differences were statistically significant (P<0.05). Additionally, there was a negative correlation between the expression levels of serum sST2 and the proportion of NK cells in moderate and severe TBI patients (r=-0.328, P<0.01). In contrast, no significant correlation is observed between these two parameters in patients with mild TBI (P>0.05). Conclusion There is a correlation between the expression levels of sST2 and NK cell in moderate and severe TBI patients, whereas no significant correlation exists between sST2 expression levels and NK cells in patients with mild TBI. |
| keywords:Soluble growth stimulation expressed gene 2 Natural killer cell Traumatic brain injury |
| 查看全文 查看/发表评论 下载PDF阅读器 |
|
|
|
