| miR-1抑制食管鳞状细胞癌细胞增殖、迁移及侵袭 |
| 投稿时间：2024-10-30 修订日期：2024-12-17 点此下载全文 |
| 引用本文：姚丽,左伟晨,苟思琪,李艺洁,王娟红.miR-1抑制食管鳞状细胞癌细胞增殖、迁移及侵袭[J].医学研究杂志,2025,54(4):84-88, 95 |
| DOI：
10.11969/j.issn.1673-548X.2025.04.016 |
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| 基金项目:陕西省重点研发计划一般项目(2024SF-YBXM-115) |
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| 中文摘要:目的 研究miR-1对食管鳞状细胞癌(esophageal squamous cell carcinoma, ESCC)细胞增殖、迁移及侵袭的影响。方法 将miR-1的模拟物(mimics)和抑制物(inhibitors)以及相应对照分别转染食管鳞状细胞癌细胞系ECa109和KYSE410,荧光标记miRNA确定最佳转染条件,荧光实时定量PCR技术评价转染效率后,用MTT法、平板克隆实验、划痕实验和Transwell体外侵袭实验检测细胞的增殖、迁移和侵袭能力。SPSS 22.0软件对数据进行统计分析。结果 上调miR-1表达量后,肿瘤细胞增殖活性下降,克隆形成能力减弱,而抑制miR-1的表达,则使肿瘤细胞的增殖速度上升,克隆形成能力增强(P<0.05)。提高miR-1表达量对肿瘤细胞的迁移及侵袭能力有抑制性作用,而降低miR-1表达量后,肿瘤细胞的迁移及侵袭能力具有显著增强(P<0.05)。结论 食管鳞状细胞癌细胞中miR-1可抑制肿瘤细胞的增殖、迁移和侵袭能力,提示miR-1在食管鳞状细胞癌的发生、发展中发挥了抑癌基因作用,可为食管鳞状细胞癌提供新的潜在治疗靶点。 |
| 中文关键词:miR-1 食管鳞状细胞癌 增殖 迁移 侵袭 |
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| MiR-1 Inhibits Proliferation, Migration and Invasion of Esophageal Squamous Cell Carcinoma. |
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| Abstract:Objective To investigate the effects of miR-1 on the proliferation, migration and invasion of esophageal squamous cell carcinoma. Methods miR-1mimics, inhibitors and their corresponding controls were transfected into ESCC cells ECa109 and KYSE410. The efficiency of cell transfection was validated by SYBR-green quantitative PCR. The proliferation, migration and invasion ability of ESCC cells were assessed by MTT, foci formation, wound healing and Matrigel invasion assays, respectively. Statistical analysis was carried out using SPSS 22.0software for Windows. Results Overexpression of miR-1markedly inhibited the cell growth rate and the colony-forming ability of the ESCC cells. While, downregulation of miR-1 promoted the cell growth rate and foci formation(P<0.05). And upregulation or downregulation of miR-1significantly suppressed or promoted tumor cell invasion and migration(P<0.05). Conclusion miR-1 inhibited ESCC cell proliferation, invasion and migration, suggesting that miR-1 functions as a tumor suppressor in the tumorigenesis and progression of ESCC and may be a potential therapeutic target. |
| keywords:miR-1 Esophageal squamous cell carcinoma Proliferation Migration Invasion |
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