| 基于生物信息学分析细胞质核糖体生物发生因子1在肝癌中的表达及意义 |
| 投稿时间：2024-10-14 修订日期：2024-11-11 点此下载全文 |
| 引用本文：梅小才,张建,曹玲玲,舒文,陈浪,陈乾.基于生物信息学分析细胞质核糖体生物发生因子1在肝癌中的表达及意义[J].医学研究杂志,2025,54(4):102-110 |
| DOI：
10.11969/j.issn.1673-548X.2025.04.019 |
| 摘要点击次数: 52 |
| 全文下载次数: 18 |
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| 基金项目:贵州省基础研究计划(自然学科类)基金资助项目[黔科合基础-ZK(2024)一般247] |
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| 中文摘要:目的 利用生物信息学方法探讨细胞质核糖体生物发生因子1(treacle ribosome biogenesis factor 1,TCOF1)在肝细胞癌中的表达及临床意义。方法 使用TCGA是数据库分析TCOF1在肝细胞癌组织和正常肝组织中的表达差异,并分析TCOF1的表达与肝细胞癌患者的临床病理参数及生存预后的关系；利用LinkedOmics在线工具分析TCOF1共表达的相关基因；利用基因富集分析探究TCOF1在肝细胞癌中的可能作用的机制。将敲低TCOF1的si-RNA转染肝癌细胞后,以平板克隆实验、EDU实验分析TCOF1抑制后对肝癌细胞增殖的影响。结果 TCOF1在肝细胞癌组织中的表达量明显高于癌旁组织(P<0.001),TCOF1的表达与肝细胞癌患者性别、年龄、肿瘤的分级、转移情况、组织学类型及TP53的突变具有相关性(P<0.05)。高表达的TCOF1的肝细胞癌患者的总体生存期(P<0.001)和无病生存期(P<0.001)较差。与NC组比较,抑制TCOF1的表达后,肝癌细胞的增殖能力明显下调。TCOF1与多种基因密切相关,进一步的富集分析展示相关基因在多种生物学过程中发挥重要作用。结论 TCOF1在肝细胞癌组织中的表达增加且导致肝细胞癌患者的不良预后,其有望成为肝细胞癌潜在的预后标志物和肿瘤治疗的新靶点。 |
| 中文关键词:TCOF1 肝细胞癌 预后 生物信息学 表达水平 临床意义 |
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| Analysis of the Expression of TCOF1 in Hepatocellular Carcinoma and its Clinical Significance Based on Bioinformatics. |
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| Abstract:Objective To investigate the expression of cytoplasmic ribosome biogenesis factor 1 (TCOF1) in hepatocellular carcinoma and its clinical significance by using bioinformatics methods.Methods To analyze the differences in TCOF1 expression in hepatocellular carcinoma tissues and normal liver tissues using TCGA is database and to analyze the relationship between TCOF1 expression and clinicopathological parameters and survival prognosis of hepatocellular carcinoma patients. The LinkedOmics online tool was used to analyze the genes associated with TCOF1 co-expression; gene enrichment analysis was used to explore the possible mechanism of the role of TCOF1 in hepatocellular carcinoma. After transfection of hepatocellular carcinoma cells with si-RNA knocking down TCOF1, the effect of TCOF1 inhibition on the proliferation of hepatocellular carcinoma cells was analysed by plate cloning assay, EDU assay. ResultsTCOF1 expression was significantly higher in hepatocellular carcinoma tissues than in paraneoplastic tissues (P<0.001). The expression of TCOF1 correlated with gender, age, tumor grade, metastasis, histological type and mutation of TP53 in hepatocellular carcinoma patients (P<0.05). Overall survival (P<0.001) and disease-free survival (P<0.001) were worse in patients with hepatocellular carcinoma with high expression of TCOF1.The proliferative ability of hepatocellular carcinoma cells was significantly down-regulated after inhibiting the expression of TCOF1 compared with the NC group.TCOF1 was closely associated with multiple genes, and further enrichment analysis demonstrated that these genes play important roles in a variety of biological processes.Conclusion TCOF1 expression is increased in hepatocellular carcinoma tissues and leads to poor prognosis in hepatocellular carcinoma patients, and it is expected to be a potential prognostic marker for hepatocellular carcinoma and a new target for tumor therapy. |
| keywords:TCOF1 Hepatocellular carcinoma Prognosis Bioinformatics Level of expression Clinical significance |
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