| 基于p38丝裂原活化蛋白激酶信号轴探讨复方金贯蓝对痤疮的影响 |
| 投稿时间：2024-10-22 修订日期：2024-11-20 点此下载全文 |
| 引用本文：刘燕丽,高军,石春蕊,崔敬,张剑虹.基于p38丝裂原活化蛋白激酶信号轴探讨复方金贯蓝对痤疮的影响[J].医学研究杂志,2025,54(4):136-140 |
| DOI：
10.11969/j.issn.1673-548X.2025.04.024 |
| 摘要点击次数: 42 |
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| 基金项目:甘肃省自然科学基金资助项目(22JR11RA043) |
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| 中文摘要:目的 研究复方金贯蓝制剂通过p38丝裂原活化蛋白激酶(mitogen-activated protein kinase,MAPK)信号通路对寻常痤疮的影响。方法 将新西兰大白兔随机分为金贯蓝高、中、低浓度组、阳性对照组、模型对照组和空白对照组,构建兔耳痤疮模型,各组分别给与相应的药物干预,观察各组兔耳皮肤组织大体变化；HE染色法观察组织病理变化；ELISA法检测血清中白细胞介素-6(interleukin-6, IL-6)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的水平；Western blot法检测兔耳组织p38 MAPK的蛋白表达水平。结果 复方金贯蓝能显著抑制寻常痤疮炎症,减轻兔耳肿胀、红斑及角化过度、炎细胞浸润；ELISA检测结果表明,与模型对照组比较,各浓度复方金贯蓝组血清中IL-6及TNF-α水平均明显降低,其中,金贯蓝高浓度组降低更明显(P＜0.01)。Western blot法结果表明,与模型组比较,复方金贯蓝组p38MAPK蛋白相对表达量降低。结论 复方金贯蓝制剂可以减缓寻常痤疮的炎性反应,其作用可能通过调控p38MAPK信号通路,降低血清中IL-6、TNF-α的表达水平实现的。 |
| 中文关键词:寻常痤疮 复方金贯蓝制剂 IL-6 TNF-α p38MAPK |
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| Exploring the Impact of Compound Jinguanlan on Acne Based on the p38 MAPK Signaling Axis. |
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| Abstract:Objective To study the effect of Compound Jinguanlan preparation on acne vulgaris through the p38MAPK signaling pathway.Methods New Zealand White Rabbits were randomly divided into high, medium, and low concentration groups of Jinguanlan, a positive control group, a model control group, and a blank control group.A rabbit ear acne model was constructed and each group was given the corresponding drug intervention.The gross changes in the skin tissue of the rabbit ears were observed. HE staining was used to observe pathological changes. ELISA was used to detect the levels of IL-6 and TNF-α in the serum. Western blot was used to detec the levels of p38MAPK protein expression. Results Jinguanlan significantly inhibited the inflammation of acne vulgaris, reducing swelling, erythema, hyperkeratosis, and inflammatory cell infiltration in the rabbit ears. ELISA results showed that compared with the model control group, the levels of IL-6 and TNF-α in the serum of each concentration of Jinguanlan group were significantly reduced, with the high concentration group showing a more pronounced decrease (P<0.01). Western blot results indicated that compared with the model group, the relative expression of p38 MAPK protein in the Jinguanlan group was reduced.Conclusion Jinguanlan can inhibit the inflammatory response of acne vulgaris,and its effect may be achieved by regulating the p38MAPK signaling pathway and reducing the expression levels of IL-6 and TNF-α in the serum. |
| keywords:Acne vulgaris Compound Jinguanlan preparation IL-6 TNF-α p38 MAPK |
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