| 白藜芦醇调控NF-κB信号通路改善骨关节炎滑膜成纤维细胞功能的实验研究 |
| 投稿时间：2024-09-26 修订日期：2024-09-30 点此下载全文 |
| 引用本文：孙文,李学艳,张力源,张语恒,张春.白藜芦醇调控NF-κB信号通路改善骨关节炎滑膜成纤维细胞功能的实验研究[J].医学研究杂志,2025,54(4):151-156 |
| DOI：
10.11969/j.issn.1673-548X.2025.04.027 |
| 摘要点击次数: 49 |
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| 基金项目:江苏省苏州市科学技术局医学应用基础研究项目(SKY2023200) |
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| 中文摘要:目的 探讨白藜芦醇(resveratrol, RES)对骨关节炎滑膜成纤维细胞(osteoarthritis-synovioblast, OA-FLS)功能的影响及分子机制。方法 将购买的原代滑膜细胞培养至第3代后,采用IL-1β诱导OA-FLS细胞模型。通过CCK-8实验观察FLS细胞活力,筛选出合适的RES干预浓度,将细胞分为正常组、模型组、低剂量组(1μmol/L RES)和高剂量组(5μmol/L RES)。通过细胞划痕实验和Transwell实验检测RES对OA-FLS迁移及侵袭能力的影响；通过流式细胞术检测RES对OA-FLS凋亡能力的影响；通过Western blot法检测RES对OA-FLS炎性细胞因子表达、NF-κB信号通路相关蛋白表达的影响。结果 CCK-8实验结果显示,10μmol/L浓度以上的RES明显抑制了FLS细胞活力,1μmol/L和5μmol/L的RES对细胞活力无明显影响；与正常组比较,模型组的迁移、侵袭能力增强,凋亡能力减弱,而RES干预后可显著逆转OA-FLS的迁移、侵袭和凋亡能力；Western blot法检测结果显示,与正常组比较,模型组炎性细胞因子水平升高,且NF-κB信号通路被激活,而采用不同浓度的RES处理OA-FLS可减少炎性细胞因子表达并抑制核因子转录。结论 RES抑制OA-FLS迁移及侵袭能力,促进其凋亡,并降低OA-FLS的炎性细胞因子表达,这一作用可能与RES抑制NF-κB信号通路有关。 |
| 中文关键词:白藜芦醇 骨关节炎 滑膜成纤维细胞 NF-κB信号通路 |
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| Effect of Resveratrol on the Function of Synovial Fibroblasts in Osteoarthritis by Regulating NF-κB Signaling Pathway. |
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| Abstract:Objective To investigate the effect of resveratrol (RES) on the function of osteoarthritis synovioblast (OA-FLS) and its molecular mechanism. Methods The purchased primary synovial cells were cultured to the third generation, and OA-FLS cell model was induced by IL-1β. The activity of FLS cells was observed by CCK-8 assay, and the appropriate RES intervention concentration was selected. The cells were divided into Control group, OA-FLS group, Low-RES group (1μmol/L) and High-RES group (5μmol/L). The effects of RES on the migration and invasion of OA-FLS were detected by cell scratch assay and Transwell assay. The effect of RES on OA-FLS apoptosis was detected by flow cytometry. The effects of RES on the expression of inflammatory factors in OA-FLS and related proteins in NF-κB signaling pathway were detected by Western blot. Results The results of CCK-8 experiment showed that the concentration of RES above 10μmol/L significantly inhibited the viability of FLS cells, and the concentration of RES above 1μmol/L and 5μmol/L had no significant effect on the viability of FLS cells. Compared with the Control group, the migration and invasion ability of OA-FLS group was enhanced, while the apoptosis ability was weakened, and the migration, invasion and apoptosis ability of OA-FLS group was significantly reversed after RES intervention. Western blot results showed that, compared with the Control group, the levels of inflammatory cytokines in OA-FLS group were increased, and the NF-κB signaling pathway was activated, while the treatment of OA-FLS with different concentrations of RES could reduce the expression of inflammatory cytokines and inhibit the transcription of nuclear factors. Conclusion RES inhibited migration and invasion of OA-FLS, promoted apoptosis, and decreased the expression of inflammatory factors of OA-FLS, which may be related to the inhibition of NF-κB signaling pathway by RES. |
| keywords:Resveratrol Osteoarthritis Synovial fibroblast NF-κB signaling pathway |
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