| 氢氧化钙骨水泥皮下诱导膜形态及成骨因子表达情况 |
| 投稿时间：2024-11-01 修订日期：2024-12-10 点此下载全文 |
| 引用本文：梁劭行,梁泽隆,郑继远,彭磊.氢氧化钙骨水泥皮下诱导膜形态及成骨因子表达情况[J].医学研究杂志,2025,54(5):40-44 |
| DOI：
10.11969/j.issn.1673-548X.2025.05.009 |
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| 基金项目:国家自然科学基金资助项目(82060347) |
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| 中文摘要:目的 探究观察新型材料氢氧化钙骨水泥在皮下的诱导膜形态及成骨因子表达情况。方法 15只4周龄雌性无特定病原体(specific pathogen free, SPF)昆明小鼠随机分为实验组、对照组、空白组(n= 5),取背部皮下切口,分离皮下组织与肌筋膜,实验组皮下放置氢氧化钙骨水泥材料,对照组放置聚甲基丙烯酸甲酯(polymethyl methacrylate, PMMA)材料,空白组仅行手术处理。6周后取材料周围皮下组织,观察病理结构,计算胶原容积分数(collagen volume fraction, CVF),检测各组组织的骨形态发生蛋白2(bone morphogenetic protein-2, BMP-2)、转化生长因子-β1(transforming growth factor-β1, TGF-β1)、血管内皮生长因子(vascular endothelial growth factor, VEGF)等成骨因子的阳性表达情况和相对表达量。结果 与空白组病理切片比较,对照组与实验组皮下组织存在富含胶原纤维、微血管和细胞的诱导膜结构,与对照组比较,实验组诱导膜结构中炎性浸润细胞、胶原含量、微血管减少,胶原纤维紊乱；比较各组CVF、成骨因子平均光密度(average optical density, AOD)及蛋白条带灰度值,空白组、实验组、对照组依次升高,各组之间比较差异有统计学意义(P<0.05)。结论 新型材料氢氧化钙骨水泥在皮下可形成诱导膜结构,但为期6周的诱导膜形态及成骨因子水平方面不及同期的PMMA诱导膜。 |
| 中文关键词:氢氧化钙骨水泥 氢氧化钙 聚甲基丙烯酸甲酯 诱导膜 |
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| Exploring the Morphology of Calcium Hydroxide-Bone Cement Subcutaneously Induced Membrane and Osteogenic Factor Expression |
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| Abstract:Objective To investigate and observe the morphology of subcutaneous induced membrane and osteogenic factor expression of the novel material calcium hydroxide-bone cement. Methods 15 4-week-old Specific Pathogen Free(SPF) female Kunming mice were randomly divided into three groups (n=5):experimental group, control group and blank group. A cut running deep under the skin was made on the back side,calcium hydroxide-bone cement was placed subcutaneously in the experimental group, the Polymethyl Methacrylate(PMMA) was placed subcutaneously in the control group, the blank group only with operation. After 6 weeks, the subcutaneous tissue surrounded the material was taken from each group to be observed the pathological structure, calculated the collagen volume fraction(CVF) and detected the positive expression and relative expression of osteogenic factors such as bone morphogenetic protein-2(BMP-2), transforming growth factor-β1(TGF-β1) and vascular endothelial growth factor(VEGF) in the subcutaneous tissues. Results Comparing with the pathological sections of the blank group, there were induced membrane structure rich in collagenous fiber, microvessel and cell in subcutaneous tissue of the control and experimental groups. Comparing with control group, there were decrease of inflammatory cells, collagen content, microvessels, and disorder of collagenous fiber in experimental group. Comparing the CVF, average optical density(AOD) of osteogenic factors and grey scale value of protein bands in each group, the blank group, experimental group and control group increased sequentially, and there was a significant difference between each group(P<0.05). Conclusion The new material, calcium hydroxide-bone cement, can form an induced membrane structure subcutaneously, but the 6-week induced membrane was not as good as the PMMA induced membrane during the same period in terms of morphology and osteogenic factor levels. |
| keywords:Calcium hydroxide-bone cement Calcium hydroxide Polymethyl methacrylate Induced membrane |
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