| 基于网络药理学和动物实验探究化痰祛湿活血方治疗非酒精性脂肪性肝炎的作用机制 |
| 投稿时间：2024-11-10 修订日期：2024-12-13 点此下载全文 |
| 引用本文：管雅捷,刘素彤,赵文霞,刘鸣昊.基于网络药理学和动物实验探究化痰祛湿活血方治疗非酒精性脂肪性肝炎的作用机制[J].医学研究杂志,2025,54(5):52-59, 51 |
| DOI：
10.11969/j.issn.1673-548X.2025.05.011 |
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| 基金项目:国家自然科学基金资助项目(81904154,82205086)；中国科学技术协会资助项目(2023QNRC001)；河南省科技攻关计划项目(242102310500,232102310438)；河南省卫健委中医药传承创新专项(2023ZXZX1162)；河南省卫生健康委员会资助项目(2022JDZX114)；河南省“双一流”创建学科中医学科学研究专项(HSRP-DFCTCM-2023-1-10) |
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| 中文摘要:目的 通过网络药理学和动物实验验证方法研究化痰祛湿活血方治疗非酒精性脂肪型肝炎(non-alcoholic steatohepatitis,NASH)的潜在作用机制。方法 通过基因表达数据库(gene expression omnibus,GEO)获得NASH差异表达基因；通过中药系统药理学数据库与分析平台(Traditional Chinese Medicines Systems Pharmacology Platform,TCMSP)获得化痰祛湿活血方药物活性成分及潜在靶点；结合疾病与药物靶标的交集得到治疗靶点,对药物作用靶点进行蛋白互作网络分析、构建药物-活性成分-靶点网络和富集分析；通过动物实验对关键通路进行验证。结果 共筛选出NASH 74个差异表达基因、化痰祛湿活血方97个活性成分和295个潜在靶点。通过交集筛选出JUN等5个基因作为关键靶点,并确定山奈酚、槲皮素等7个活性成分。富集分析揭示高级糖基化终末产物-受体(advanced glycation end products-the receptor of advanced glycation endproducts,AGE-RAGE)和白细胞介素-17(interleukin-17,IL-17)通路可能在化痰祛湿活血方治疗NASH中发挥关键作用。动物实验发现化痰祛湿活血方可降低总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、血糖(glucose,GLU)水平,下调AGE-RAGE和IL-17通路中RAGE和激活蛋白-1(activator protein-1,AP-1)的表达,从而发挥对NASH的治疗作用。结论 化痰祛湿活血方可通过降低TC、TG、GLU水平,抑制RAGE和AP-1蛋白的表达治疗NASH。 |
| 中文关键词:化痰祛湿活血方 非酒精性脂肪性肝炎 RAGE AP-1 |
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| Exploring the Mechanism of Huatan Qushi Huoxue Recipe in the Treatment of NASH Based on Network Pharmacology and Animal Experiments. |
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| Abstract:Objective To investigate the potential mechanisms of action of Huatan Qushi Huoxue Recipe in treating non-alcoholic steatohepatitis (NASH) through network pharmacology and animal experiments. Methods The differentially expressed genes in NASH were obtained from the GEO database. The active components and potential targets of Huatan Qushi Huoxue Recipe were obtained from the TCMSP. The treatment targets were obtained by intersecting the diseases and drug targets. The protein-protein interaction network was analyzed, the drug-active component-target network was constructed, and the enrichment analysis was performed. The key pathways were verified by animal experiments. Results A total of 74differentially expressed genes, 97 active components, and 295 potential targets were identified in NASH. Five genes, including JUN, were selected as key targets through intersection. Seven active components, including kaempferol and quercetin, were identified. Enrichment analysis revealed that the AGE-RAGE and IL-17 pathways may play a key role in the treatment of NASH by Huatan Qushi Huoxue Recipe. Animal experiments showed that Huatan Qushi Huoxue Recipe could reduce the levels of total cholesterol (TC), triglyceride (TG), and blood glucose (GLU), and down-regulate the expression of RAGE and activator protein 1 (AP-1) in the AGE-RAGE and IL-17 pathways, thus, exerting a therapeutic effect on NASH. ConclusionHuatan Qushi Huoxue Recipe can treat NASH by lowering TC, TG and GLU levels and inhibiting the expression of RAGE and AP-1 protein. |
| keywords:Huatan Qushi Huoxue recipe NASH RAGE AP-1 |
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